验证数据展示
经过测试的应用
| Positive IF-P detected in | mouse skin tissue |
| Positive IF/ICC detected in | A431 cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Immunofluorescence (IF)-P | IF-P : 1:50-1:500 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
CL594-60320 targets Cytokeratin 14 in IF/ICC, IF-P applications and shows reactivity with human, mouse samples.
| 经测试应用 | IF/ICC, IF-P Application Description |
| 经测试反应性 | human, mouse |
| 免疫原 |
CatNo: Ag17559 Product name: Recombinant human KRT14 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 426-472 aa of BC002690 Sequence: LSSSQFSSGSQSSRDVTSSSRQIRTKVMDVHDGKVVSTHEQVLRTKN 种属同源性预测 |
| 宿主/亚型 | Mouse / IgG1 |
| 抗体类别 | Monoclonal |
| 产品类型 | Antibody |
| 全称 | keratin 14 |
| 别名 | KRT14, Cytokeratin-14, Cytokeratin,Keratin, CK-14, CK14 |
| 计算分子量 | 472 aa, 52 kDa |
| 观测分子量 | 52 kDa |
| GenBank蛋白编号 | BC002690 |
| 基因名称 | Cytokeratin 14 |
| Gene ID (NCBI) | 3861 |
| RRID | AB_2934710 |
| 偶联类型 | CoraLite®594 Fluorescent Dye |
| 最大激发/发射波长 | 588 nm / 604 nm |
| 形式 | Liquid |
| 纯化方式 | Protein G purification |
| UNIPROT ID | P02533 |
| 储存缓冲液 | PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3. |
| 储存条件 | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
eratins are a large family of proteins that form the intermediate filament cytoskeleton of epithelial cells, which are classified into two major sequence types. Type I keratins are a group of acidic intermediate filament proteins, including K9-K23, and the hair keratins Ha1-Ha8. Type II keratins are the basic or neutral courterparts to the acidic type I keratins, including K1-K8, and the hair keratins, Hb1-Hb6. Keratin 14 is a type I cytokeratin. It is usually found as a heterotetramer with keratin 5. Keratins K14 and K5 have long been considered to be biochemical markers of the stratified squamous epithelia, including epidermis.
实验方案
| Product Specific Protocols | |
|---|---|
| IF protocol for CL594 Cytokeratin 14 antibody CL594-60320 | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
