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验证数据展示

  • WB analysis using 18343-1-AP (same clone as 18343-1-PBS)

    WB analysis using 18343-1-AP (same clone as 18343-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 18343-1-AP (Cytokeratin 10 antibody) at dilution of 1:200000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 18343-1-PBS in a different storage buffer formulation.

  • IHC staining of human cervical cancer using 18343-1-AP (same clone as 18343-1-PBS)

    IHC staining of human cervical cancer using 18343-1-AP (same clone as 18343-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 18343-1-AP (Cytokeratin 10 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 18343-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with KRT10 antibody included
  • IHC staining of human cervical cancer using 18343-1-AP (same clone as 18343-1-PBS)

    IHC staining of human cervical cancer using 18343-1-AP (same clone as 18343-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 18343-1-AP (Cytokeratin 10 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 18343-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with KRT10 antibody included
  • IF Staining of mouse skin using 18343-1-AP (same clone as 18343-1-PBS)

    IF Staining of mouse skin using 18343-1-AP (same clone as 18343-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse skin tissue using Cytokeratin 10 antibody (18343-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 18343-1-PBS in a different storage buffer formulation.

  • IF Staining of mouse skin using 18343-1-AP (same clone as 18343-1-PBS)

    IF Staining of mouse skin using 18343-1-AP (same clone as 18343-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse skin tissue using Cytokeratin 10 antibody (18343-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 18343-1-PBS in a different storage buffer formulation.

  • IF Staining of mouse skin using 18343-1-AP (same clone as 18343-1-PBS)

    IF Staining of mouse skin using 18343-1-AP (same clone as 18343-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse skin tissue using Cytokeratin 10 antibody (18343-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 18343-1-PBS in a different storage buffer formulation.

  • IF Staining of A431 using 18343-1-AP (same clone as 18343-1-PBS)

    IF Staining of A431 using 18343-1-AP (same clone as 18343-1-PBS)

    Immunofluorescent analysis of (-20°C Methanol) fixed A431 cells using Cytokeratin 10 antibody (18343-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 18343-1-PBS in a different storage buffer formulation.

  • FC experiment of A431 using 18343-1-AP (same clone as 18343-1-PBS)

    FC experiment of A431 using 18343-1-AP (same clone as 18343-1-PBS)

    1X10^6 A431 cells were intracellularly stained with 0.4 ug Anti-Human Cytokeratin 10 (18343-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug rabbit IgG isotype control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer. This data was developed using the same antibody clone with 18343-1-PBS in a different storage buffer formulation.

Cytokeratin 10 Polyclonal antibody, PBS Only

Cytokeratin 10 Polyclonal Antibody for WB, IHC, IF/ICC, IF-P, FC (Intra), ELISA
Cat No. 18343-1-PBS

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, IF/ICC, IF-P, FC (Intra), ELISA

别名

KRT10, CK 10, CK10, CK-10, Cytokeratin-10

缓冲液配方: 
PBS, Azide, Glycerol
偶联物:  Unconjugated
规格价格库存

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Planning an IHC experiment? We recommend our IHCeasy KRT10 Ready-To-Use IHC Kit. KRT10 primary antibody included.

产品信息

18343-1-PBS targets Cytokeratin 10 in WB, IHC, IF/ICC, IF-P, FC (Intra), ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IF/ICC, IF-P, FC (Intra), ELISA Application Description
经测试反应性 human, mouse, rat
免疫原

CatNo: Ag13136

Product name: Recombinant human KRT10 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 235-584 aa of BC034697

Sequence: RLKYENEVALRQSVEADINGLRRVLDELTLTKADLEMQIESLTEELAYLKKNHEEEMKDLRNVSTGDVNVEMNAAPGVDLTQLLNNMRSQYEQLAEQNRKDAEAWFNEKSKELTTEIDNNIEQISSYKSEITELRRNVQALEIELQSQLALKQSLEASLAETEGRYCVQLSQIQAQISALEEQLQQIRAETECQNTEYQQLLDIKIRLENEIQTYRSLLEGEGSSGGGGRGGGSFGGGYGGGSSGGGSSGGGYGGGHGGSSGGGYGGGSSGGGSSGGGYGGGSSSGGHGGSSSGGYGGGSSGGGGGGYGGGSSGGGSSSGGGYGGGSSSGGHKSSSSGSVGESSSKGPRY

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 keratin 10
别名 KRT10, CK 10, CK10, CK-10, Cytokeratin-10
计算分子量 584 aa, 59 kDa
观测分子量50-59 kDa
GenBank蛋白编号BC034697
基因名称 Cytokeratin 10
Gene ID (NCBI) 3858
RRIDAB_10863654
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDP13645
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Keratins are a large family of proteins that form the intermediate filament cytoskeleton of epithelial cells, which are classified into two major sequence types. Type I keratins are a group of acidic intermediate filament proteins, including K9-K23, and the hair keratins Ha1-Ha8. Type II keratins are the basic or neutral courterparts to the acidic type I keratins, including K1-K8, and the hair keratins, Hb1-Hb6. As a type I keratin, keratin 10 is a suprabasal marker of differentiation in stratified squamous epithelia.

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