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验证数据展示

  • WB analysis using 18343-1-AP

    WB analysis using 18343-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 18343-1-AP (Cytokeratin 10 antibody) at dilution of 1:200000 incubated at room temperature for 1.5 hours.

  • IHC staining of human cervical cancer using 18343-1-AP

    IHC staining of human cervical cancer using 18343-1-AP

    Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 18343-1-AP (Cytokeratin 10 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with KRT10 antibody included
  • IHC staining of human cervical cancer using 18343-1-AP

    IHC staining of human cervical cancer using 18343-1-AP

    Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 18343-1-AP (Cytokeratin 10 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with KRT10 antibody included
  • IF Staining of mouse skin using 18343-1-AP

    IF Staining of mouse skin using 18343-1-AP

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse skin tissue using Cytokeratin 10 antibody (18343-1-AP) at dilution of 1:200 and CoraLite®594-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-4). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of A431 using 18343-1-AP

    IF Staining of A431 using 18343-1-AP

    Immunofluorescent analysis of (-20°C Methanol) fixed A431 cells using Cytokeratin 10 antibody (18343-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2).

  • FC experiment of A431 using 18343-1-AP

    FC experiment of A431 using 18343-1-AP

    1X10^6 A431 cells were intracellularly stained with 0.4 ug Anti-Human Cytokeratin 10 (18343-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug rabbit IgG isotype control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer.

Cytokeratin 10 Polyclonal antibody

Cytokeratin 10 Polyclonal Antibody for WB, IHC, IF/ICC, IF-P, FC (Intra), ELISA
Cat No. 18343-1-AP

产品说明书

引用文献(19)

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, IF/ICC, IF-P, FC (Intra), ELISA and More (1)

别名

KRT10, CK 10, CK10, CK-10, Cytokeratin-10

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
偶联物:  Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inmouse skin tissue, A431 cells, rat skin tissue
Positive IHC detected inhuman cervical cancer tissue, human breast cancer tissue, human lung cancer tissue, human skin cancer tissue, rat skin tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF-P detected inmouse skin tissue
Positive IF/ICC detected inA431 cells
Positive FC (Intra) detected inA431 cells
Planning an IHC experiment? We recommend our IHCeasy KRT10 Ready-To-Use IHC Kit. KRT10 primary antibody included.
Planning an IF experiment? We recommend our CoraLite® Plus 555 and CoraLite® Plus 647 conjugated versions of this antibody.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:5000-1:20000
Immunohistochemistry (IHC)IHC : 1:500-1:2000
Immunofluorescence (IF)-PIF-P : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

18343-1-AP targets Cytokeratin 10 in WB, IHC, IF/ICC, IF-P, FC (Intra), ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IF/ICC, IF-P, FC (Intra), ELISA Application Description
文献引用应用WB, IHC, IF
经测试反应性 human, mouse, rat
文献引用反应性human, mouse, rat
免疫原

CatNo: Ag13136

Product name: Recombinant human KRT10 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 235-584 aa of BC034697

Sequence: RLKYENEVALRQSVEADINGLRRVLDELTLTKADLEMQIESLTEELAYLKKNHEEEMKDLRNVSTGDVNVEMNAAPGVDLTQLLNNMRSQYEQLAEQNRKDAEAWFNEKSKELTTEIDNNIEQISSYKSEITELRRNVQALEIELQSQLALKQSLEASLAETEGRYCVQLSQIQAQISALEEQLQQIRAETECQNTEYQQLLDIKIRLENEIQTYRSLLEGEGSSGGGGRGGGSFGGGYGGGSSGGGSSGGGYGGGHGGSSGGGYGGGSSGGGSSGGGYGGGSSSGGHGGSSSGGYGGGSSGGGGGGYGGGSSGGGSSSGGGYGGGSSSGGHKSSSSGSVGESSSKGPRY

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 keratin 10
别名 KRT10, CK 10, CK10, CK-10, Cytokeratin-10
计算分子量 584 aa, 59 kDa
观测分子量50-59 kDa
GenBank蛋白编号BC034697
基因名称 Cytokeratin 10
Gene ID (NCBI) 3858
RRIDAB_10863654
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDP13645
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Keratins are a large family of proteins that form the intermediate filament cytoskeleton of epithelial cells, which are classified into two major sequence types. Type I keratins are a group of acidic intermediate filament proteins, including K9-K23, and the hair keratins Ha1-Ha8. Type II keratins are the basic or neutral courterparts to the acidic type I keratins, including K1-K8, and the hair keratins, Hb1-Hb6. As a type I keratin, keratin 10 is a suprabasal marker of differentiation in stratified squamous epithelia.

实验方案

Product Specific Protocols
FC protocol for Cytokeratin 10 antibody 18343-1-APDownload protocol
IF protocol for Cytokeratin 10 antibody 18343-1-APDownload protocol
IHC protocol for Cytokeratin 10 antibody 18343-1-APDownload protocol
WB protocol for Cytokeratin 10 antibody 18343-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
ratIF

Cell Mol Biol Lett

The role of NPY2R/NFATc1/DYRK1A regulatory axis in sebaceous glands for sebum synthesis

Authors - Tao Yang
View Article
mouseIF

Aging Cell

Nociceptive transient receptor potential canonical 7 (TRPC7) mediates aging-associated tumorigenesis induced by ultraviolet B.

Authors - Wen-Li Hsu
View Article
mouseIHC

Life Sci

The potential of Diosgenin in treating psoriasis: Studies from HaCaT keratinocytes and imiquimod-induced murine model.

Authors - Shidi Wu
View Article
humanIF

mBio

Commensal-Related Changes in the Epidermal Barrier Function Lead to Alterations in the Benzo[a]Pyrene Metabolite Profile and Its Distribution in 3D Skin.

Authors - Lisa Lemoine
View Article
humanIF

Eur Surg Res

A novel combination of keratinocyte and autologous microskin grafting to repair full thickness skin loss

Authors - Kai Yin
View Article
mouseIF

Int J Biol Macromol

Skin-adaptive film dressing with smart-release of growth factors accelerated diabetic wound healing

Authors - Yingzheng Zhao
View Article
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