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验证数据展示

  • WB analysis using 82681-1-RR (same clone as 82681-1-PBS)

    WB analysis using 82681-1-RR (same clone as 82681-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 82681-1-RR (Cyclin D1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 82681-1-PBS in a different storage buffer formulation.

  • IHC staining of human oesophagus cancer using 82681-1-RR (same clone as 82681-1-PBS)

    IHC staining of human oesophagus cancer using 82681-1-RR (same clone as 82681-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human oesophagus cancer tissue slide using 82681-1-RR (Cyclin D1 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 82681-1-PBS in a different storage buffer formulation.

  • IHC staining of human tonsillitis using 82681-1-RR (same clone as 82681-1-PBS)

    IHC staining of human tonsillitis using 82681-1-RR (same clone as 82681-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 82681-1-RR (Cyclin D1 antibody) at dilution of 1:1000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 82681-1-PBS in a different storage buffer formulation.

  • Sandwich ELISA standard curve of MP80003-1

    Sandwich ELISA standard curve of MP80003-1

    Sandwich ELISA standard curve of MP80003-1, Cyclin D1 Hybrid Matched Antibody Pair, PBS Only. Capture antibody: 68790-1-PBS. Detection antibody: 82681-1-PBS. Standard: Ag25384. Range: 0.188-12 ng/mL.

  • IF Staining of MCF-7 using 82681-1-RR (same clone as 82681-1-PBS)

    IF Staining of MCF-7 using 82681-1-RR (same clone as 82681-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed MCF-7 cells using Cyclin D1 antibody (82681-1-RR, Clone: 6B10 ) at dilution of 1:2000 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-phalloidin (red). This data was developed using the same antibody clone with 82681-1-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 82681-1-RR

    Affinity and Kinetic Characterization of 82681-1-RR

    Biolayer interferometry (BLl) kinetic assays of 82681-1-RR against Human Cyclin D1 were performed. The affinity constant is 0.153 nM.

Cyclin D1 Recombinant antibody, PBS Only (Detector)

Cyclin D1 Uni-rAbTM Recombinant Antibody for WB, IHC, IF/ICC, Sandwich ELISA, Indirect ELISA, Sample test
Cat No. 82681-1-PBS

产品说明书

CloneNo. 6B10

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, IF/ICC, Sandwich ELISA, Indirect ELISA, Sample test

别名

CCND1, 6B10, B cell lymphoma 1 protein, B-cell lymphoma 1 protein, BCL-1

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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产品信息

82681-1-PBS targets Cyclin D1 as part of a matched antibody pair:

MP80003-1: 68790-1-PBS capture and 82681-1-PBS detection (validated in Sandwich ELISA)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

经测试应用 WB, IHC, IF/ICC, Sandwich ELISA, Indirect ELISA, Sample test Application Description
经测试反应性 human, mouse, rat
免疫原 Cyclin D1 fusion protein Ag25384 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 cyclin D1
别名 CCND1, 6B10, B cell lymphoma 1 protein, B-cell lymphoma 1 protein, BCL-1
计算分子量 295 aa, 34 kDa
观测分子量 34 kDa
GenBank蛋白编号BC000076
基因名称 Cyclin D1
Gene ID (NCBI) 595
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDP24385
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

CCND1 (cyclin D1), also known as PRAD1 or BCL1, belongs to the highly conserved cyclin family, whose members are characterized by a dramatic periodicity in protein abundance throughout the cell cycle. CCND1 forms a complex with and functions as a regulatory subunit of CDK4 or CDK6, whose activity is required for cell cycle G1/S transition. The CCND1 gene, located on 11q13 has been reported to be overexpressed in mantle cell lymphoma (MCL) due to the chromosomal translocation. CCND1 has been shown to interact with tumor suppressor protein Rb and the expression of this gene is regulated positively by Rb. Over-expression of CCND1 is known to correlate with the early onset of cancer and risk of tumor progression and metastasis.