验证数据展示
经过测试的应用
| Positive WB detected in | HEK-293 cells, HeLa cells, U2OS cells, Jurkat cells |
Planning an IHC experiment? We recommend our IHCeasy CCNA2 Ready-To-Use IHC Kit. CCNA2 primary antibody included.
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| WB | See 6 publications below |
产品信息
27242-1-AP targets Cyclin A2 in WB, ELISA applications and shows reactivity with human samples.
| 经测试应用 | WB, ELISA Application Description |
| 文献引用应用 | WB |
| 经测试反应性 | human |
| 文献引用反应性 | human |
| 免疫原 |
CatNo: Ag25666 Product name: Recombinant human Cyclin A2 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 405-432 aa of BC104783 Sequence: AQQSIREKYKNSKYHGVSLLNPPETLNL 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | cyclin A2 |
| 别名 | CCN1, CCNA, CCNA2, Cyclin A, Cyclin A2, cyclinA2 |
| 计算分子量 | 432 aa, 49 kDa |
| 观测分子量 | 49 kDa |
| GenBank蛋白编号 | BC104783 |
| 基因名称 | Cyclin A2 |
| Gene ID (NCBI) | 890 |
| RRID | AB_2880815 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | P20248 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for Cyclin A2 antibody 27242-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Cancer Sci Methylation of NRN1 is a novel synthetic lethal marker of PI3K-Akt-mTOR and ATR inhibitors in esophageal cancer. | ||
Int J Mol Med Morusin inhibits the growth of human colorectal cancer HCT116‑derived sphere‑forming cells via the inactivation of Akt pathway. | ||
Biomed Pharmacother Deapioplatycodin D promotes cell senescence induced by P21 through the mediation of incomplete mitophagy via BNIP3L | ||
Biochem Biophys Res Commun Constructing and validating pan-apoptosis-related features for predicting prognosis and immunotherapy response in hepatocellular carcinoma | ||
Oncogenesis CENPF (+) cancer cells promote malignant progression of early-stage TP53 mutant lung adenocarcinoma |
