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验证数据展示

  • WB analysis using 22184-1-AP

    WB analysis using 22184-1-AP

    mouse heart tissue was subjected to SDS PAGE followed by western blot with 22184-1-AP (Connexin 45 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • IHC staining of mouse brain using 22184-1-AP

    IHC staining of mouse brain using 22184-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 22184-1-AP (Connexin 45 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Featured Product
  • KD/KO验证

Connexin 45 Polyclonal antibody

Connexin 45 Polyclonal Antibody for WB, IHC, ELISA
Cat No. 22184-1-AP

产品说明书

引用文献(1)

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse and More (1)

应用

WB, IHC, ELISA

别名

Connexin-45, Cx45, Gap junction alpha-7 protein, Gap junction gamma-1 protein, GJA7

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inmouse heart tissue
Positive IHC detected inmouse brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:1000-1:4000
Immunohistochemistry (IHC)IHC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 1 publications below

产品信息

22184-1-AP targets Connexin 45 in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, IHC, ELISA Application Description
文献引用应用WB
经测试反应性 human, mouse
文献引用反应性rat
免疫原

CatNo: Ag16742

Product name: Recombinant human GJC1 protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 248-396 aa of BC096213

Sequence: WEMLHLGFGTIRDSLNSKRRELEDPGAYNYPFTWNTPSAPPGYNIAVKPDQIQYTELSNAKIAYKQNKANTAQEQQYGSHEENLPADLEALQREIRMAQERLDLAVQAYSHQNNPHGPREKKAKVGSKAGSNKSTASSKSGDGKNSVWI

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 gap junction protein, gamma 1, 45kDa
别名 Connexin-45, Cx45, Gap junction alpha-7 protein, Gap junction gamma-1 protein, GJA7
计算分子量 396 aa, 45 kDa
观测分子量45 kDa
GenBank蛋白编号BC096213
基因名称 Connexin-45
Gene ID (NCBI) 10052
RRIDAB_3085686
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDP36383
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Connexin 45 (Cx45), also known as Gap junction gamma protein (GJC1), belongs to the connexin family. Connexins are membrane-spanning proteins that assemble to form gap junction channels that facilitate the transfer of ions and small molecules between cells. A loss-of-function mutation in Cx45 contributing to Atrial fibrillation (AF) and other cardiac arrhythmias (PMID: 33429106).

实验方案

Product Specific Protocols
IHC protocol for Connexin 45 antibody 22184-1-APDownload protocol
WB protocol for Connexin 45 antibody 22184-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
ratWB

Appl Biochem Biotechnol

Cardiac Fibroblasts Enhance MMP2 Activity to Suppress Gap Junction Function in Cardiomyocytes

Authors - Xue Bai
View Article
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