验证数据展示
经过测试的应用
| Positive WB detected in | mouse brain tissue, rat brain tissue, pig brain tissue, rat cerebellum tissue |
| Positive IHC detected in | mouse heart tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF-P detected in | mouse heart tissue |
| Positive IF/ICC detected in | HeLa cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| Immunohistochemistry (IHC) | IHC : 1:1000-1:4000 |
| Immunofluorescence (IF)-P | IF-P : 1:50-1:500 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:125-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
80543-1-RR targets Connexin 43 in WB, IHC, IF/ICC, IF-P, ELISA applications and shows reactivity with human, mouse, rat, pig samples.
| 经测试应用 | WB, IHC, IF/ICC, IF-P, ELISA Application Description |
| 经测试反应性 | human, mouse, rat, pig |
| 免疫原 | Connexin 43 fusion protein Ag25533 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Recombinant |
| 产品类型 | Antibody |
| 全称 | gap junction protein, alpha 1, 43kDa |
| 别名 | GJA1, Connexin-43, 3O6, Gap junction alpha-1 protein, Gap junction alpha 1 protein |
| 计算分子量 | 43 kDa |
| 观测分子量 | 43 kDa |
| GenBank蛋白编号 | BC026329 |
| 基因名称 | Connexin-43 |
| Gene ID (NCBI) | 2697 |
| RRID | AB_2935545 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Protein A purification |
| UNIPROT ID | P17302 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Connexin 43 (Cx43, also known as gap junction alpha-1), a member of connexin family, plays essential roles in gap junction communication that facilitates direct communication among adjacent cells (PMID: 12270943). Usually, six connexin proteins oligomerize into a hemi-channel or connexon during intercellular channel formation (PMID: 12270943). Mutations of Connexin 43 may cause a series of diseases such as oculodentodigital dysplasia (ODDD), syndactyly 3 (SDTY3), hypoplastic left heart syndrome 1 (HLHS1) and so on (PMID: 18161618, 1472936, 1147490).
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for Connexin 43 antibody 80543-1-RR | Download protocol |
| IHC protocol for Connexin 43 antibody 80543-1-RR | Download protocol |
| IF protocol for Connexin 43 antibody 80543-1-RR | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |