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验证数据展示

  • WB analysis using 17251-1-AP (same clone as 17251-1-PBS)

    WB analysis using 17251-1-AP (same clone as 17251-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 17251-1-AP (Collagen Type VIII antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 17251-1-PBS in a different storage buffer formulation.

  • IP experiment of HepG2 using 17251-1-AP (same clone as 17251-1-PBS)

    IP experiment of HepG2 using 17251-1-AP (same clone as 17251-1-PBS)

    IP result of anti-Collagen Type VIII (IP:17251-1-AP, 4ug; Detection:17251-1-AP 1:500) with HepG2 cells lysate 1800ug. This data was developed using the same antibody clone with 17251-1-PBS in a different storage buffer formulation.

  • IF Staining of HepG2 using 17251-1-AP (same clone as 17251-1-PBS)

    IF Staining of HepG2 using 17251-1-AP (same clone as 17251-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using COL8A1 antibody (17251-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 17251-1-PBS in a different storage buffer formulation.

  • IF Staining of HepG2 using 17251-1-AP (same clone as 17251-1-PBS)

    IF Staining of HepG2 using 17251-1-AP (same clone as 17251-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using COL8A1 antibody (17251-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 17251-1-PBS in a different storage buffer formulation.

  • Featured Product
  • KD/KO Validated

COL8A1 Polyclonal antibody, PBS Only

COL8A1 Polyclonal Antibody for WB, IF/ICC, IP, Indirect ELISA
Cat No. 17251-1-PBS

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IF/ICC, IP, Indirect ELISA

别名

COL8, C3orf7, Collagen alpha-1(VIII) chain, Collagen Type VIII

缓冲液配方:  PBS Only
PBS, Azide, Glycerol
偶联物:  Unconjugated
规格价格库存

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产品信息

17251-1-PBS targets COL8A1 in WB, IF/ICC, IP, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IF/ICC, IP, Indirect ELISA Application Description
经测试反应性 human, mouse, rat
免疫原

CatNo: Ag10607

Product name: Recombinant human COL8A1 protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 396-744 aa of BC013581

Sequence: GIPGPMGPPGAIGFPGPKGEGGIVGPQGPPGPKGEPGLQGFPGKPGFLGEVGPPGMRGLPGPIGPKGEAGQKGVPGLPGVPGLLGPKGEPGIPGDQGLQGPPGIPGIGGPSGPIGPPGIPGPKGEPGLPGPPGFPGIGKPGVAGLHGPPGKPGALGPQGQPGLPGPPGPPGPPGPPAVMPPTPPPQGEYLPDMGLGIDGVKPPHAYGAKKGKNGGPAYEMPAFTAELTAPFPPVGAPVKFNKLLYNGRQNYNPQTGIFTCEVPGVYYFAYHVHCKGGNVWVALFKNNEPVMYTYDEYKKGFLDQASGSAVLLLRPGDRVFLQMPSEQAAGLYAGQYVHSSFSGYLLYPM

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 collagen, type VIII, alpha 1
别名 COL8, C3orf7, Collagen alpha-1(VIII) chain, Collagen Type VIII
计算分子量 744 aa, 73 kDa
观测分子量 73 kDa
GenBank蛋白编号BC013581
基因名称 COL8A1
Gene ID (NCBI) 1295
RRIDAB_10696173
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDP27658
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C
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