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COL8A1 Polyclonal antibody

COL8A1 Polyclonal Antibody for WB, IF/ICC, IP, ELISA
Cat No. 17251-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat and More (1)

应用

WB, IF/ICC, IP, ELISA and More (2)

COL8, C3orf7, Collagen alpha-1(VIII) chain, Collagen Type VIII

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格价格库存


经过测试的应用

Positive WB detected inA375 cells, HeLa cells, HepG2 cells, COLO 320 cells, mouse kidney tissue, mouse spleen tissue, rat kidney tissue
Positive IP detected inHepG2 cells
Positive IF/ICC detected inHepG2 cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:3000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunofluorescence (IF)/ICCIF/ICC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

17251-1-AP targets COL8A1 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IF/ICC, IP, ELISA Application Description
文献引用应用WB, IHC, IF
经测试反应性 human, mouse, rat
文献引用反应性human, mouse, rat, canine
免疫原 COL8A1 fusion protein Ag10607 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 collagen, type VIII, alpha 1
别名 COL8, C3orf7, Collagen alpha-1(VIII) chain, Collagen Type VIII
计算分子量 744 aa, 73 kDa
观测分子量 73 kDa
GenBank蛋白编号BC013581
基因名称 COL8A1
Gene ID (NCBI) 1295
RRIDAB_10696173
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDP27658
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for COL8A1 antibody 17251-1-APDownload protocol
IF protocol for COL8A1 antibody 17251-1-APDownload protocol
IP protocol for COL8A1 antibody 17251-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
mouseIF

Sci China Life Sci

Identification of LRRC46 as a novel candidate gene for high myopia

Authors - Lingxi Jiang
humanWB

Front Bioeng Biotechnol

Let-7i-5p Regulation of Cell Morphology and Migration Through Distinct Signaling Pathways in Normal and Pathogenic Urethral Fibroblasts.

Authors - Kaile Zhang
ratIF

Front Cell Dev Biol

Platelet-Derived Extracellular Vesicles Increase Col8a1 Secretion and Vascular Stiffness in Intimal Injury.

Authors - Han Bao
  • KD Validated
mouseWB

FASEB J

Silencing of METTL3 attenuates cardiac fibrosis induced by myocardial infarction via inhibiting the activation of cardiac fibroblasts.

Authors - Tingting Li
ratWB

Biomed Pharmacother

Compound Danshen Dripping Pill inhibits doxorubicin or isoproterenol-induced cardiotoxicity.

Authors - Ke Feng
mouseWB,IF

Dis Model Mech

Upregulation of distinct collagen transcripts in post-surgery scar tissue: a study of conjunctival fibrosis.

Authors - Li-Fong Seet
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