验证数据展示
经过测试的应用
| Positive WB detected in | mouse ovary tissue, mouse liver tissue |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
24148-1-AP targets COL9A2 in WB, ELISA applications and shows reactivity with mouse samples.
| 经测试应用 | WB, ELISA Application Description |
| 经测试反应性 | mouse |
| 免疫原 |
CatNo: Ag21458 Product name: Recombinant human COL9A2 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 278-406 aa of BC136326 Sequence: EKGDEGSPGIRGPQGITGPKGATGPPGINGKDGTPGTPGMKGSAGQAGQPGSPGHQGLAGVPGQPGTKGGPGDQGEPGPQGLPGFSGPPGKEGEPGPRGEIGPQGIMGQKGDQGERGPVGQPGPQGRQG 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | collagen, type IX, alpha 2 |
| 别名 | Collagen alpha 2(IX) chain, Collagen alpha-2(IX) chain, Collagen IX, Collagen Type IX, EDM2 |
| 计算分子量 | 689 aa, 65 kDa |
| 观测分子量 | 68 kDa |
| GenBank蛋白编号 | BC136326 |
| 基因名称 | COL9A2 |
| Gene ID (NCBI) | 1298 |
| RRID | AB_3085726 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen Affinity purified |
| UNIPROT ID | Q14055 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
This gene encodes one of the three alpha chains of type IX collagen, the major collagen component of hyaline cartilage. Type IX collagen, a heterotrimeric molecule, is usually found in tissues containing type II collagen, a fibrillar collagen. This chain is unusual in that, unlike the other two type IX alpha chains, it contains a covalently attached glycosaminoglycan side chain. Mutations in this gene are associated with multiple epiphyseal dysplasia.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for COL9A2 antibody 24148-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
