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验证数据展示

  • WB analysis of pig colon using 67288-1-Ig (same clone as 67288-1-PBS)

    WB analysis of pig colon using 67288-1-Ig (same clone as 67288-1-PBS)

    pig colon tissue were subjected to SDS PAGE followed by western blot with 67288-1-Ig (Collagen Type I antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67288-1-PBS in a different storage buffer formulation.

  • IHC staining of human breast cancer using 67288-1-Ig (same clone as 67288-1-PBS)

    IHC staining of human breast cancer using 67288-1-Ig (same clone as 67288-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 67288-1-Ig (Collagen Type I antibody) at dilution of 1:5000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67288-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with COL1A1 antibody included
  • IHC staining of human breast cancer using 67288-1-Ig (same clone as 67288-1-PBS)

    IHC staining of human breast cancer using 67288-1-Ig (same clone as 67288-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 67288-1-Ig (Collagen Type I antibody) at dilution of 1:5000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67288-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with COL1A1 antibody included
  • IF Staining of human colon cancer using 67288-1-Ig (same clone as 67288-1-PBS)

    IF Staining of human colon cancer using 67288-1-Ig (same clone as 67288-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed human colon cancer tissue using Collagen Type I antibody (67288-1-Ig, Clone: 1E9A7 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67288-1-PBS in a different storage buffer formulation.

  • IF Staining of human colon cancer using 67288-1-Ig (same clone as 67288-1-PBS)

    IF Staining of human colon cancer using 67288-1-Ig (same clone as 67288-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed human colon cancer tissue using Collagen Type I antibody (67288-1-Ig, Clone: 1E9A7 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67288-1-PBS in a different storage buffer formulation.

  • WB analysis using 67288-1-Ig (same clone as 67288-1-PBS)

    WB analysis using 67288-1-Ig (same clone as 67288-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 67288-1-Ig (Collagen Type I antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67288-1-PBS in a different storage buffer formulation.

  • IHC staining of human colon cancer using 67288-1-Ig (same clone as 67288-1-PBS)

    IHC staining of human colon cancer using 67288-1-Ig (same clone as 67288-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 67288-1-Ig (Collagen Type I antibody) at dilution of 1:4000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67288-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with COL1A1 antibody included

Collagen Type I Monoclonal antibody, PBS Only

Collagen Type I Monoclonal Antibody for WB, IHC, IF-P, Indirect ELISA
Cat No. 67288-1-PBS

产品说明书

CloneNo. 1E9A7

宿主/亚型

Mouse / IgG1

种属反应性

human, pig

应用

WB, IHC, IF-P, Indirect ELISA

别名

COL1A1, 1E9A7, Alpha 1 type I collagen, Alpha-1 type I collagen, COL I

缓冲液配方:  PBS Only
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
规格价格库存

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Planning an IHC experiment? We recommend our IHCeasy COL1A1 Ready-To-Use IHC Kit. COL1A1 primary antibody included.

产品信息

67288-1-PBS targets Collagen Type I in WB, IHC, IF-P, Indirect ELISA applications and shows reactivity with human, pig samples.

经测试应用 WB, IHC, IF-P, Indirect ELISA Application Description
经测试反应性 human, pig
免疫原

Peptide

种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 collagen, type I, alpha 1
别名 COL1A1, 1E9A7, Alpha 1 type I collagen, Alpha-1 type I collagen, COL I
计算分子量139 kDa
观测分子量100-140 kDa，200kDa
GenBank蛋白编号NM_000088
基因名称 COL1A1
Gene ID (NCBI) 1277
RRIDAB_2882554
偶联类型 Unconjugated
形式Liquid
纯化方式Protein G purification
UNIPROT IDP02452
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Type I collagen, the major structural component of connective tissues such as skin, tendon and bone, is the most abundant and widely expressed collagen in humans (PMID: 7620364; 8645190; 9016532). Type I collagen is a heterotrimer comprising one alpha 2(I) and two alpha 1(I) chains which are encoded by the unlinked loci COL1A2 and COL1A1 respectively. Mutations in COL1A1 are associated with osteogenesis imperfecta types I-IV, Ehlers-Danlos syndrome type VIIA, Ehlers-Danlos syndrome Classical type, Caffey Disease and idiopathic osteoporosis. This antibody raised against a synthesized peptide corresponding to 1206-1218 aa of human pro-alpha 1 chain of type I collagen recognize collagen alpha-1(I) chain. The presence of unprocessed, intermediate, and mature chains of type I collagen was clearly detected only in static constructs. Indeed, in sponges cultured under perfusion the presence of type I collagen was mainly restricted to mature chains, suggesting that HACs were no longer actively producing type I collagen (PMID: 27584727). It is reported that the molecular weight is 200kDa(PMID: 28526934).

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