Collagen Type I Monoclonal antibody

Collagen Type I Monoclonal Antibody for WB, IHC, IF-P, ELISA
Cat No. 67288-1-Ig

产品说明书

CloneNo. 1E9A7

宿主/亚型

Mouse / IgG1

种属反应性

human, pig and More (2)

应用

WB, IHC, IF-P, ELISA and More (2)

COL1A1, 1E9A7, Alpha 1 type I collagen, Alpha-1 type I collagen, COL I

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格价格库存


经过测试的应用

Positive WB detected inpig colon tissue, human cervical cancer tissue, human placenta tissue, MG-63 cells, pig lung tissue, pig skin tissue
Positive IHC detected inhuman breast cancer tissue, human colon tissue, human colon cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF-P detected inhuman colon cancer tissue
Planning an IHC experiment? We recommend our IHCeasy COL1A1 Ready-To-Use IHC Kit. COL1A1 primary antibody included.
Planning an IF experiment? We recommend our CoraLite® Plus 488 and CoraLite®594 conjugated versions of this antibody.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:2500-1:10000
Immunofluorescence (IF)-PIF-P : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

67288-1-Ig targets Collagen Type I in WB, IHC, IF-P, CoIP, ELISA applications and shows reactivity with human, pig samples.

经测试应用 WB, IHC, IF-P, ELISA Application Description
文献引用应用WB, IHC, IF, CoIP
经测试反应性 human, pig
文献引用反应性human, pig, rabbit, zebrafish
免疫原 Peptide 种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 collagen, type I, alpha 1
别名 COL1A1, 1E9A7, Alpha 1 type I collagen, Alpha-1 type I collagen, COL I
计算分子量139 kDa
观测分子量100-140 kDa,200kDa
GenBank蛋白编号NM_000088
基因名称 COL1A1
Gene ID (NCBI) 1277
RRIDAB_2882554
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein G purification
UNIPROT IDP02452
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Type I collagen, the major structural component of connective tissues such as skin, tendon and bone, is the most abundant and widely expressed collagen in humans (PMID: 7620364; 8645190; 9016532). Type I collagen is a heterotrimer comprising one alpha 2(I) and two alpha 1(I) chains which are encoded by the unlinked loci COL1A2 and COL1A1 respectively. Mutations in COL1A1 are associated with osteogenesis imperfecta types I-IV, Ehlers-Danlos syndrome type VIIA, Ehlers-Danlos syndrome Classical type, Caffey Disease and idiopathic osteoporosis. This antibody raised against a synthesized peptide corresponding to 1206-1218 aa of human pro-alpha 1 chain of type I collagen recognize collagen alpha-1(I) chain. The presence of unprocessed, intermediate, and mature chains of type I collagen was clearly detected only in static constructs. Indeed, in sponges cultured under perfusion the presence of type I collagen was mainly restricted to mature chains, suggesting that HACs were no longer actively producing type I collagen (PMID: 27584727). It is reported that the molecular weight is 200kDa(PMID: 28526934).

实验方案

Product Specific Protocols
WB protocol for Collagen Type I antibody 67288-1-IgDownload protocol
IHC protocol for Collagen Type I antibody 67288-1-IgDownload protocol
IF protocol for Collagen Type I antibody 67288-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
pigIF

Adv Mater

Biodegradable Piezoelectric-Conductive Integrated Hydrogel Scaffold for Repair of Osteochondral Defects

Authors - Dingge Liu
humanIHC

J Hematol Oncol

Proteogenomic insights into the biology and treatment of pancreatic ductal adenocarcinoma

Authors - Yexin Tong
humanIF

Nat Cell Biol

Persistence of a regeneration-associated, transitional alveolar epithelial cell state in pulmonary fibrosis.

Authors - Yoshihiko Kobayashi
humanIHC

Adv Sci (Weinh)

Silencing of NOTCH3 Signaling in Meniscus Smooth Muscle Cells Inhibits Fibrosis and Exacerbates Degeneration in a HEYL-Dependent Manner

Authors - Hao Sun
WB,IHC

Small

M2 Macrophage Membrane-Mediated Biomimetic-Nanoparticle Carrying COX-siRNA Targeted Delivery for Prevention of Tendon Adhesions by Inhibiting Inflammation

Authors - Jie Sun
WB

Eur Respir J

Intracellular hydroxyproline imprinting following resolution of bleomycin-induced pulmonary fibrosis.

Authors - Shengren Song
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