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验证数据展示

  • WB analysis using 88053-1-RR

    WB analysis using 88053-1-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 88053-1-RR (Cofilin antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • IHC staining of human appendix using 88053-1-RR

    IHC staining of human appendix using 88053-1-RR

    Immunohistochemical analysis of paraffin-embedded human appendix tissue slide using 88053-1-RR (Cofilin antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with CFL1 antibody included

Cofilin Recombinant monoclonal antibody

Cofilin Uni-rAb® Recombinant Antibody for WB, IHC, ELISA
Cat No. 88053-1-RR

产品说明书

CloneNo. 260317G7

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, ELISA

别名

CFL1, 18 kDa phosphoprotein, Cofilin 1, Cofilin, non-muscle isoform, Cofilin1

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inA431 cells, HeLa cells, LNCaP cells, Jurkat cells, HEK-293 cells, Neuro-2a cells, PC-12 cells
Positive IHC detected inhuman appendix tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Planning an IHC experiment? We recommend our IHCeasy CFL1 Ready-To-Use IHC Kit. CFL1 primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

88053-1-RR targets Cofilin in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, ELISA Application Description
经测试反应性 human, mouse, rat
免疫原

CatNo: Ag1399

Product name: Recombinant human Cofilin protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-166 aa of BC012318

Sequence: MASGVAVSDGVIKVFNDMKVRKSSTPEEVKKRKKAVLFCLSEDKKNIILEEGKEILVGDVGQTVDDPYATFVKMLPDKDCRYALYDATYETKESKKEDLVFIFWAPESAPLKSKMIYASSKDAIKKKLTGIKHELQANCYEEVKDRCTLAEKLGGSAVISLEGKPL

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 cofilin 1 (non-muscle)
别名 CFL1, 18 kDa phosphoprotein, Cofilin 1, Cofilin, non-muscle isoform, Cofilin1
计算分子量 18 kDa
观测分子量 18 kDa
GenBank蛋白编号BC012318
基因名称 Cofilin
Gene ID (NCBI) 1072
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purification
UNIPROT IDP23528
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Cofilin is a ubiquitous actin-binding protein required for the reorganization of actin filaments. It is a member of ADF (actin-depolymerizing factor)/cofilin family that is a key regulator of actin dynamics and essential for cellular motility, cytokinesis, and endocytosis. Cofilin activity is tightly regulated by phosphorylation and dephosphorylation.Phosphorylation at Ser3 can inhibit its activity, also causing translocation from the nucleus to the cytoplasm.

实验方案

Product Specific Protocols
IHC protocol for Cofilin antibody 88053-1-RRDownload protocol
WB protocol for Cofilin antibody 88053-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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