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验证数据展示

  • WB analysis using 66057-1-Ig (same clone as 66057-1-PBS)

    WB analysis using 66057-1-Ig (same clone as 66057-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 66057-1-Ig (Cofilin antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66057-1-PBS in a different storage buffer formulation.

  • IHC staining of human testis using 66057-1-Ig (same clone as 66057-1-PBS)

    IHC staining of human testis using 66057-1-Ig (same clone as 66057-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human testis using 66057-1-Ig(CFL1 antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 66057-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with CFL1 antibody included
  • IHC staining of human testis using 66057-1-Ig (same clone as 66057-1-PBS)

    IHC staining of human testis using 66057-1-Ig (same clone as 66057-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human testis using 66057-1-Ig(CFL1 antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 66057-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with CFL1 antibody included
  • WB analysis using 66057-1-Ig (same clone as 66057-1-PBS)

    WB analysis using 66057-1-Ig (same clone as 66057-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 66057-1-Ig (Cofilin antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66057-1-PBS in a different storage buffer formulation.

  • IF Staining of HeLa using 66057-1-Ig (same clone as 66057-1-PBS)

    IF Staining of HeLa using 66057-1-Ig (same clone as 66057-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using Cofilin antibody (66057-1-Ig, Clone: 1G6A2 ) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) (SA00013-1), CL594-phalloidin (red). This data was developed using the same antibody clone with 66057-1-PBS in a different storage buffer formulation.

  • Featured Product
  • KD/KO验证

Cofilin Monoclonal antibody, PBS Only

Cofilin Monoclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA
Cat No. 66057-1-PBS

产品说明书

CloneNo. 1G6A2

宿主/亚型

Mouse / IgG2a

种属反应性

human, mouse, rat, pig, rabbit

应用

WB, IHC, IF/ICC, Indirect ELISA

别名

CFL1, Cofilin1, Cofilin, non-muscle isoform, Cofilin 1, 1G6A2

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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Planning an IHC experiment? We recommend our IHCeasy CFL1 Ready-To-Use IHC Kit. CFL1 primary antibody included.

产品信息

66057-1-PBS targets Cofilin in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse, rat, pig, rabbit samples.

经测试应用 WB, IHC, IF/ICC, Indirect ELISA Application Description
经测试反应性 human, mouse, rat, pig, rabbit
免疫原 Cofilin fusion protein Ag18044 种属同源性预测
宿主/亚型 Mouse / IgG2a
抗体类别 Monoclonal
产品类型 Antibody
全称 cofilin 1 (non-muscle)
别名 CFL1, Cofilin1, Cofilin, non-muscle isoform, Cofilin 1, 1G6A2
计算分子量 18 kDa
观测分子量 18 kDa
GenBank蛋白编号BC012318
基因名称 Cofilin
Gene ID (NCBI) 1072
RRIDAB_11043339
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDP23528
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Cofilin is a ubiquitous actin-binding protein required for the reorganization of actin filaments. It is a member of ADF (actin-depolymerizing factor)/cofilin family that is a key regulator of actin dynamics and essential for cellular motility, cytokinesis, and endocytosis. Cofilin activity is tightly regulated by phosphorylation and dephosphorylation. Phosphorylation at Ser3 can inhibit its activity, also causing translocation from the nucleus to the cytoplasm.