Cleaved PARP1 (Asp214) Recombinant antibody, PBS Only

Cleaved PARP1 (Asp214) Uni-rAbTM Recombinant Antibody for WB, Indirect ELISA
Cat No. 85175-5-PBS

产品说明书

CloneNo. 243142B10

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, Indirect ELISA

PARP1, ADP-ribosyltransferase diphtheria toxin-like 1, ADPRT, ADPRT 1, ADPRT1

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格价格库存


Planning an IHC experiment? We recommend our IHCeasy PARP1 Ready-To-Use IHC Kit. PARP1 primary antibody included.

产品信息

85175-5-PBS targets Cleaved PARP1 (Asp214) in WB, Indirect ELISA applications and shows reactivity with human samples.

经测试应用 WB, Indirect ELISA Application Description
经测试反应性 human
免疫原 Peptide 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 poly (ADP-ribose) polymerase 1
别名 PARP1, ADP-ribosyltransferase diphtheria toxin-like 1, ADPRT, ADPRT 1, ADPRT1
计算分子量 1014 aa, 113 kDa
观测分子量89 kDa
GenBank蛋白编号BC037545
基因名称 PARP1
Gene ID (NCBI) 142
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDP09874
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

PARP1 (poly(ADP-ribose) polymerase 1) is a nuclear enzyme catalyzing the poly(ADP-ribosyl)ation of many key proteins in vivo. The normal function of PARP1 is the routine repair of DNA damage. Activated by DNA strand breaks, the PARP1 is cleaved into an 85 to 89-kDa COOH-terminal fragment and a 24 kDa NH2-terminal peptide by caspases during the apoptotic process. The appearance of PARP fragments is commonly considered an important biomarker of apoptosis. In addition to caspases, other proteases like calpains, cathepsins, granzymes, and matrix metalloproteinases (MMPs) have also been reported to cleave PARP1 and give rise to fragments ranging from 42-89 kDa.
This antibody only recognizes the cleaved form of PAPR1 but not full-length PARP1.

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