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验证数据展示

  • WB analysis of Jurkat using 87055-4-RR (same clone as 87055-4-PBS)

    WB analysis of Jurkat using 87055-4-RR (same clone as 87055-4-PBS)

    Staurosporine treated Jurkat cells were subjected to SDS PAGE followed by western blot with 87055-4-RR (Cleaved Caspase 3/P12 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with GAPDH Monoclonal antibody (60004-1-Ig) as loading control. This data was developed using the same antibody clone with 87055-4-PBS in a different storage buffer formulation.

  • IF Staining of Jurkat using 87055-4-RR (same clone as 87055-4-PBS)

    IF Staining of Jurkat using 87055-4-RR (same clone as 87055-4-PBS)

    Immunofluorescent analysis of (4% PFA) fixed Staurosporine treated Jurkat cells using Cleaved Caspase 3/P12 antibody (87055-4-RR, Clone: 252012D12 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 87055-4-PBS in a different storage buffer formulation.

Cleaved Caspase 3/P12 Recombinant monoclonal antibody, PBS Only

Cleaved Caspase 3/P12 Uni-rAb® Recombinant Antibody for WB, IF/ICC, Indirect ELISA
Cat No. 87055-4-PBS

产品说明书

CloneNo. 252012D12

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IF/ICC, Indirect ELISA

别名

Cleaved Caspase 3 P12, Cleaved Caspase-3 P12, Cleaved Caspase-3/P12, CASP3, Caspase3

缓冲液配方:  PBS Only
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
规格价格库存

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Planning an IHC experiment? We recommend our IHCeasy Cleaved Caspase 3 Ready-To-Use IHC Kit. Cleaved Caspase 3 primary antibody included.

产品信息

87055-4-PBS targets Cleaved Caspase 3/P12 in WB, IF/ICC, Indirect ELISA applications and shows reactivity with human samples.

经测试应用 WB, IF/ICC, Indirect ELISA Application Description
经测试反应性 human
免疫原

Peptide

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 caspase 3, apoptosis-related cysteine peptidase
别名 Cleaved Caspase 3 P12, Cleaved Caspase-3 P12, Cleaved Caspase-3/P12, CASP3, Caspase3
计算分子量 277 aa, 32 kDa
观测分子量12 kDa
GenBank蛋白编号BC016926
基因名称 Caspase 3
Gene ID (NCBI) 836
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purification
UNIPROT IDP42574
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Cleaved Caspase-3 p12 is one of the two subunits of active Caspase-3 and plays a key catalytic role in the execution of apoptosis. Precursor cleavage of Caspase-3 ultimately forms an active heterotetramer composed of two p17 subunits and two p12 subunits.

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