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验证数据展示

  • WB analysis of Jurkat using 87055-4-RR

    WB analysis of Jurkat using 87055-4-RR

    Staurosporine treated Jurkat cells were subjected to SDS PAGE followed by western blot with 87055-4-RR (Cleaved Caspase 3/P12 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with GAPDH Monoclonal antibody (60004-1-Ig) as loading control.

  • IF Staining of Jurkat using 87055-4-RR

    IF Staining of Jurkat using 87055-4-RR

    Immunofluorescent analysis of (4% PFA) fixed Staurosporine treated Jurkat cells using Cleaved Caspase 3/P12 antibody (87055-4-RR, Clone: 252012D12 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

Cleaved Caspase 3/P12 Recombinant monoclonal antibody

Cleaved Caspase 3/P12 Uni-rAb® Recombinant Antibody for WB, IF/ICC, ELISA
Cat No. 87055-4-RR

产品说明书

CloneNo. 252012D12

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IF/ICC, ELISA

别名

Cleaved Caspase 3 P12, Cleaved Caspase-3 P12, Cleaved Caspase-3/P12, CASP3, Caspase3

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inStaurosporine treated Jurkat cells
Positive IF/ICC detected inStaurosporine treated Jurkat cells
Planning an IHC experiment? We recommend our IHCeasy Cleaved Caspase 3 Ready-To-Use IHC Kit. Cleaved Caspase 3 primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:2000-1:10000
Immunofluorescence (IF)/ICCIF/ICC : 1:400-1:1600
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

87055-4-RR targets Cleaved Caspase 3/P12 in WB, IF/ICC, ELISA applications and shows reactivity with human samples.

经测试应用 WB, IF/ICC, ELISA Application Description
经测试反应性 human
免疫原

Peptide

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 caspase 3, apoptosis-related cysteine peptidase
别名 Cleaved Caspase 3 P12, Cleaved Caspase-3 P12, Cleaved Caspase-3/P12, CASP3, Caspase3
计算分子量 277 aa, 32 kDa
观测分子量12 kDa
GenBank蛋白编号BC016926
基因名称 Caspase 3
Gene ID (NCBI) 836
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purification
UNIPROT IDP42574
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Cleaved Caspase-3 p12 is one of the two subunits of active Caspase-3 and plays a key catalytic role in the execution of apoptosis. Precursor cleavage of Caspase-3 ultimately forms an active heterotetramer composed of two p17 subunits and two p12 subunits.

实验方案

Product Specific Protocols
IF protocol for Cleaved Caspase 3/P12 antibody 87055-4-RRDownload protocol
WB protocol for Cleaved Caspase 3/P12 antibody 87055-4-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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