验证数据展示
经过测试的应用
| Positive WB detected in | Staurosporine treated Jurkat cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:4000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
88209-2-RR targets Cleaved Caspase 2/P12 in WB, ELISA applications and shows reactivity with human samples.
| 经测试应用 | WB, ELISA Application Description |
| 经测试反应性 | human |
| 免疫原 |
Peptide 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Recombinant |
| 产品类型 | Antibody |
| 全称 | caspase 2, apoptosis-related cysteine peptidase |
| 别名 | CASP2, Caspase 2, CASP 2, CASP-2, Caspase 2/p18 |
| 计算分子量 | 452 aa, 51 kDa |
| 观测分子量 | 12 kDa |
| GenBank蛋白编号 | BC002427 |
| 基因名称 | Caspase 2 |
| Gene ID (NCBI) | 835 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Protein A purification |
| UNIPROT ID | P42575 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
CASP2(Caspase-2) is also named as ICH1, NEDD2 and belongs to the peptidase C14A family.It is involved in the activation cascade of caspases responsible for apoptosis execution and might function by either activating some proteins required for cell death or inactivating proteins necessary for cell survival. Caspase 2 is synthesized in its inactive prozymogen form and is activated upon stimulation by apoptosis signals. During activation, the prozymogen is cleaved at a specific site, producing a smaller p12 subunit and a larger p18 subunit. The p12-p18 tetramer structure is the core form in which Caspase 2 functions.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for Cleaved Caspase 2/P12 antibody 88209-2-RR | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
