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验证数据展示

  • IHC staining of human oesophagus cancer using 29795-1-AP (same clone as 29795-1-PBS)

    IHC staining of human oesophagus cancer using 29795-1-AP (same clone as 29795-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human oesophagus cancer tissue slide using 29795-1-AP (Claudin 7 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 29795-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with CLDN7 antibody included
  • IHC staining of human oesophagus cancer using 29795-1-AP (same clone as 29795-1-PBS)

    IHC staining of human oesophagus cancer using 29795-1-AP (same clone as 29795-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human oesophagus cancer tissue slide using 29795-1-AP (Claudin 7 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 29795-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with CLDN7 antibody included
  • IHC staining of human colon using 29795-1-AP (same clone as 29795-1-PBS)

    IHC staining of human colon using 29795-1-AP (same clone as 29795-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 29795-1-AP (Claudin 7 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 29795-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with CLDN7 antibody included
  • IHC staining of human colon using 29795-1-AP (same clone as 29795-1-PBS)

    IHC staining of human colon using 29795-1-AP (same clone as 29795-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 29795-1-AP (Claudin 7 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 29795-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with CLDN7 antibody included
  • WB analysis using 29795-1-AP (same clone as 29795-1-PBS)

    WB analysis using 29795-1-AP (same clone as 29795-1-PBS)

    Rat colon tissue was subjected to SDS PAGE followed by western blot with 29795-1-AP (Claudin 7 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 29795-1-PBS in a different storage buffer formulation.

  • IF Staining of mouse colon using 29795-1-AP (same clone as 29795-1-PBS)

    IF Staining of mouse colon using 29795-1-AP (same clone as 29795-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse colon tissue using Claudin 7 antibody (29795-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 29795-1-PBS in a different storage buffer formulation.

Claudin 7 Polyclonal antibody, PBS Only

Claudin 7 Polyclonal Antibody for WB, IHC, IF-P, Indirect ELISA
Cat No. 29795-1-PBS

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, IHC, IF-P, Indirect ELISA

别名

CEPTRL2, CLDN 7, CLDN7, CLDN-7, CPETRL2

缓冲液配方: 
PBS, Azide, Glycerol
偶联物:  Unconjugated
规格价格库存

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Planning an IHC experiment? We recommend our IHCeasy CLDN7 Ready-To-Use IHC Kit. CLDN7 primary antibody included.

产品信息

29795-1-PBS targets Claudin 7 in WB, IHC, IF-P, Indirect ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, IHC, IF-P, Indirect ELISA Application Description
经测试反应性 human, mouse
免疫原

CatNo: Ag31706

Product name: Recombinant human CLDN7 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 150-211 aa of BC001055

Sequence: NPLIPTNIKYEFGPAIFIGWAGSALVILGGALLSCSCPGNESKAGYRAPRSYPKSNSSKEYV

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 claudin 7
别名 CEPTRL2, CLDN 7, CLDN7, CLDN-7, CPETRL2
计算分子量22.4 kDa
观测分子量23 kDa
GenBank蛋白编号BC001055
基因名称 Claudin 7
Gene ID (NCBI) 1366
RRIDAB_2935481
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDO95471
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Claudins are a family of proteins that are the most important components of the tight junctions, where they establish the paracellular barrier that controls the flow of molecules in the intercellular space between the cells of an epithelium. 27 claudins have been identified. They are small (20-27 kilodalton (kDa)) proteins with very similar structure. They have four transmembrane domains, with the N-terminus and the C-terminus in the cytoplasm.

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