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Claudin 7 Polyclonal antibody

Claudin 7 Polyclonal Antibody for IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human

Applications

IHC, ELISA

Conjugate

Unconjugated

Cat No : 29795-1-AP

Print datasheet

Synonyms

CEPTRL2, Claudin 7, CLDN 7, CLDN7, CPETRL2, Hs.84359

验证数据展示

  • IHC staining of human oesophagus cancer using 29795-1-AP

    IHC staining of human oesophagus cancer using 29795-1-AP

    Immunohistochemical analysis of paraffin-embedded human oesophagus cancer tissue slide using 29795-1-AP (Claudin 7 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with CLDN7 antibody included
  • IHC staining of human oesophagus cancer using 29795-1-AP

    IHC staining of human oesophagus cancer using 29795-1-AP

    Immunohistochemical analysis of paraffin-embedded human oesophagus cancer tissue slide using 29795-1-AP (Claudin 7 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with CLDN7 antibody included
  • IHC staining of human colon using 29795-1-AP

    IHC staining of human colon using 29795-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 29795-1-AP (Claudin 7 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with CLDN7 antibody included
  • IHC staining of human colon using 29795-1-AP

    IHC staining of human colon using 29795-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 29795-1-AP (Claudin 7 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with CLDN7 antibody included

经过测试的应用

Positive IHC detected inhuman colon tissue, mouse colon tissue, human oesophagus cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Planning an IHC experiment? We recommend our IHCeasy CLDN7 Ready-To-Use IHC Kit. CLDN7 primary antibody included.

推荐稀释比

ApplicationDilution
Immunohistochemistry (IHC)IHC : 1:250-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

29795-1-AP targets Claudin 7 in IHC, ELISA applications and shows reactivity with Human samples.

Tested Applications IHC, ELISA
Tested Reactivity Human
Immunogen Claudin 7 fusion protein Ag31706 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name claudin 7
Synonyms CEPTRL2, Claudin 7, CLDN 7, CLDN7, CPETRL2, Hs.84359
Calculated Molecular Weight22.4 kDa
Observed Molecular Weight23 kDa
GenBank Accession NumberBC001055
Gene Symbol CLDN7
Gene ID (NCBI) 1366
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDO95471
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Claudins are a family of proteins that are the most important components of the tight junctions, where they establish the paracellular barrier that controls the flow of molecules in the intercellular space between the cells of an epithelium. 27 claudins have been identified. They are small (20-27 kilodalton (kDa)) proteins with very similar structure. They have four transmembrane domains, with the N-terminus and the C-terminus in the cytoplasm.

实验方案

Product Specific Protocols
IHC protocol for Claudin 7 antibody 29795-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols