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CoraLite® Plus 488-conjugated Chromogranin A Polyclonal antibody

Chromogranin A Polyclonal Antibody for FC (Intra), IF-P

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

FC (Intra), IF-P

Conjugate

CoraLite® Plus 488 Fluorescent Dye

Cat No : CL488-10529

Print datasheet

Synonyms

CGA, CHGA, Chromogranin A, Pituitary secretory protein I, SP I, Vasostatin I Vasostatin 2

验证数据展示

  • IF Staining of mouse pancreas using CL488-10529

    IF Staining of mouse pancreas using CL488-10529

    Immunofluorescent analysis of (4% PFA) fixed mouse pancreas tissue using CoraLite® Plus 488 Chromogranin A antibody (CL488-10529) at dilution of 1:200.

  • IF Staining of mouse pancreas using CL488-10529

    IF Staining of mouse pancreas using CL488-10529

    Immunofluorescent analysis of (4% PFA) fixed mouse pancreas tissue using CoraLite® Plus 488 Chromogranin A antibody (CL488-10529) at dilution of 1:200.

  • FC experiment of Neuro-2a using CL488-10529

    FC experiment of Neuro-2a using CL488-10529

    1X10^6 Neuro-2a cells were intracellularly stained with 0.4 ug CoraLite® Plus 488 Anti-Human Chromogranin A (CL488-10529) (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

经过测试的应用

Positive IF detected inmouse pancreas tissue
Positive FC detected inNeuro-2a cells
Planning an IHC experiment? We recommend our IHCeasy CgA/CHGA Ready-To-Use IHC Kit. CgA/CHGA primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 10529-1-AP

推荐稀释比

ApplicationDilution
Immunofluorescence (IF)IF : 1:50-1:500
Flow Cytometry (FC)FC : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-10529 targets Chromogranin A in IF, FC (Intra) applications and shows reactivity with human, mouse, rat samples.

Tested Applications FC (Intra), IF-P
Tested Reactivity human, mouse, rat
Immunogen Chromogranin A fusion protein Ag0807 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name chromogranin A (parathyroid secretory protein 1)
Synonyms CGA, CHGA, Chromogranin A, Pituitary secretory protein I, SP I, Vasostatin I Vasostatin 2
Calculated Molecular Weight 51 kDa
GenBank Accession NumberBC006459
Gene Symbol CHGA
Gene ID (NCBI) 1113
RRIDAB_2883077
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission Maxima Wavelengths493 nm / 522 nm
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP10645
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Aliquoting is unnecessary for -20oC storage.

背景介绍

Chromogranin A is a member of the granin family of neuroendocrine secretory proteins. It is located in secretory vesicles of neurons and endocrine cells. Chromogranin A is the precursor to several functional peptides including vasostatin, pancreastatin, catestatin and parastatin. These peptides negatively modulate the neuroendocrine function of the releasing cell (autocrine) or nearby cells (paracrine). CgA is one of the most used tumor markers in NET's (neuroendocrine tumors) , and elevated CgA concentrations have been demonstrated in serum or plasma of patients with different types of these tumors.

实验方案

Product Specific Protocols
IF protocol for CL Plus 488 Chromogranin A antibody CL488-10529Download protocol
FC protocol for CL Plus 488 Chromogranin A antibody CL488-10529Download protocol
Standard Protocols
Click here to view our Standard Protocols