验证数据展示
经过测试的应用
| Positive WB detected in | Chicken egg |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
HRP-67405 targets Chicken IgY in WB applications and shows reactivity with Chicken samples.
| 经测试应用 | WB Application Description |
| 经测试反应性 | Chicken |
| 免疫原 | Peptide 种属同源性预测 |
| 宿主/亚型 | Mouse / IgG2a |
| 抗体类别 | Monoclonal |
| 产品类型 | Antibody |
| 全称 | Chicken IgY |
| 别名 | |
| 观测分子量 | 180 kDa, 70 kDa |
| 基因名称 | |
| Gene ID (NCBI) | |
| RRID | AB_2883846 |
| 偶联类型 | HRP |
| 形式 | Liquid |
| 纯化方式 | Protein A purification |
| 储存缓冲液 | PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA , pH 7.3 |
| 储存条件 | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
IgY (immunoglobulin Y) is a type of immunoglobulin found in birds and reptiles. High concentration of IgY exists in chicken egg yolk. In chickens, IgY is functional same to IgG. IgY antibodies do not cross-react with Fc receptors, mammalian immunoglobulins such as rheumatoid factors and HAMA or activate the complement system thus could reduce false positive results in immunological assays. This antibody was generated from chicken IgY that extracted from chicken eggs. This product recognizes the heavry chain of checken IgY and can detect the contact form of chicken IgY (~180 kDa) and reduced form (~70 kDa).
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for HRP Chicken IgY antibody HRP-67405 | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |