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验证数据展示

  • IF Staining of hTERT-RPE1 using CL594-12794

    IF Staining of hTERT-RPE1 using CL594-12794

    Immunofluorescent analysis of (-20°C Methanol) fixed hTERT-RPE1 cells using CoraLite®594 Centrin 1 antibody (CL594-12794) at dilution of 1:200.

  • IF Staining of HeLa using CL594-12794

    IF Staining of HeLa using CL594-12794

    Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using CoraLite®594 Centrin 1 antibody (CL594-12794) at dilution of 1:200.

CoraLite®594-conjugated Centrin 1 Polyclonal antibody

Centrin 1 Polyclonal Antibody for IF/ICC
Cat No. CL594-12794

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

IF/ICC

别名

CETN1, CETN, Centrin-1, CEN1, Caltractin isoform 2

缓冲液配方:  PBS, Proclin 300, BSA, Glycerol
PBS, Proclin 300, BSA, Glycerol
偶联物:  CoraLite®594
规格价格库存

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经过测试的应用

Positive IF/ICC detected inhTERT-RPE1 cells, HeLa cells
For other applications, we recommend the unconjugated version of this antibody, 12794-1-AP

推荐稀释比

应用推荐稀释比
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL594-12794 targets Centrin 1 in IF/ICC applications and shows reactivity with human samples.

经测试应用 IF/ICC Application Description
经测试反应性 human
免疫原

CatNo: Ag3529

Product name: Recombinant human CETN1 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-172 aa of BC029515

Sequence: MASGFKKPSAASTGQKRKVAPKPELTEDQKQEVREAFDLFDVDGSGTIDAKELKVAMRALGFEPRKEEMKKMISEVDREGTGKISFNDFLAVMTQKMSEKDTKEEILKAFRLFDDDETGKISFKNLKRVANELGENLTDEELQEMIDEADRDGDGEVNEEEFLRIMKKTSLY

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 centrin, EF-hand protein, 1
别名 CETN1, CETN, Centrin-1, CEN1, Caltractin isoform 2
计算分子量 172 aa, 20 kDa
GenBank蛋白编号BC029515
基因名称 Centrin 1
Gene ID (NCBI) 1068
RRIDAB_2919788
偶联类型 CoraLite®594 Fluorescent Dye
最大激发/发射波长588 nm / 604 nm
形式Liquid
纯化方式Antigen Affinity Purified
UNIPROT IDQ12798
储存缓冲液 PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
储存条件Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

EF-hand type Ca2+-binding proteins consists of several family members, including Centrin-1, Centrin-2 and Centrin-3. The Centrin proteins are ubiquitously expressed cytoskeletal components that show increased expression during cell differentiation. Centrin-1 plays important roles in the determination of centrosome position and segregation, and in the process of microtubule severing. Centrin-1 is localized to the centrosome of interphase cells, and redistributes to the region of the spindle poles during mitosis, reflecting the dynamic behavior of the centrosome during the cell cycle.

实验方案

Product Specific Protocols
IF protocol for CL594 Centrin 1 antibody CL594-12794Download protocol
Standard Protocols
Click here to view our Standard Protocols
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