验证数据展示

WB analysis of RAW 264.7 using 28058-1-AP
RAW 264.7 cells were subjected to SDS PAGE followed by western blot with 28058-1-AP (CD68 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.
IHC staining of mouse spleen using 28058-1-AP
Immunohistochemical analysis of paraffin-embedded mouse spleen tissue slide using 28058-1-AP (CD68 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
Ready-to-use IHC kits available with CD68 antibody included
IHC staining of mouse spleen using 28058-1-AP
Immunohistochemical analysis of paraffin-embedded mouse spleen tissue slide using 28058-1-AP (CD68 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
Ready-to-use IHC kits available with CD68 antibody included
IF Staining of human tonsillitis using 28058-1-AP
Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded human tonsillitis tissue using CD68 antibody (28058-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IF Staining of human tonsillitis using 28058-1-AP
Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded human tonsillitis tissue using CD68 antibody (28058-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

CD68 Polyclonal antibody

CD68 Polyclonal Antibody for WB, IHC, IF-P, ELISA

Cat No. 28058-1-AP

产品说明书

引用文献(316)

宿主/亚型

Rabbit / IgG

种属反应性

mouse, rat and More (2)

应用

WB, IHC, IF-P, ELISA and More (1)

别名

CD68 antigen, gp110, Scard1

缓冲液配方: PBS, Azide, Glycerol

PBS, Azide, Glycerol

偶联物: Unconjugated

Unconjugated

CoraLite® Plus 488

规格	价格	库存

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概述

经过测试的应用
产品信息
发表文章 (316)
实验方案

产品说明书

MSDS

经过测试的应用

Positive WB detected in	RAW 264.7 cells, mouse spleen tissue, NR8383 cells
Positive IHC detected in	mouse spleen tissue, mouse liver tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***
Positive IF-P detected in	human tonsillitis tissue

Planning an IHC experiment? We recommend our IHCeasy CD68 Ready-To-Use IHC Kit. CD68 primary antibody included.

Planning an IF experiment? We recommend our CoraLite® Plus 488 conjugated versions of this antibody.

应用	推荐稀释比
Western Blot (WB)	WB : 1:600-1:2000
Immunohistochemistry (IHC)	IHC : 1:1000-1:4000
Immunofluorescence (IF)-P	IF-P : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WB	See 60 publications below
IHC	See 124 publications below
IF	See 154 publications below

产品信息

28058-1-AP targets CD68 in WB, IHC, IF-P, ELISA applications and shows reactivity with mouse, rat samples.

经测试应用	WB, IHC, IF-P, ELISA Application Description
文献引用应用	WB, IHC, IF
经测试反应性	mouse, rat
文献引用反应性	mouse, rat, pig, canine
免疫原	CatNo: Ag27827 Product name: Recombinant mouse Cd68 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 119-291 aa of NM_001291058 Sequence: MPSPRSKGALGNYTWANGSQPCVQLQAQIQIRILYPIQGGRKAWGISVLNPNKTKVQGGCDGTHPHLSLSFPYGQLTFGFKQDLHQSPSTVYLDYMAVEYNVSFPQAAQWTFMAQNSSLRELQAPLGQSFCCGNASIVLSPAVHLDLLSLRLQAAQLPDKGHFGPCFSCNRDQS 种属同源性预测
宿主/亚型	Rabbit / IgG
抗体类别	Polyclonal
产品类型	Antibody
全称	CD68 antigen
别名	CD68 antigen, gp110, Scard1
计算分子量	35 kDa
观测分子量	100 kDa
GenBank蛋白编号	NM_001291058
基因名称	Cd68
Gene ID (NCBI)	12514
RRID	AB_2881049
偶联类型	Unconjugated
形式	Liquid
纯化方式	Antigen affinity purification
UNIPROT ID	P31996
储存缓冲液	PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

背景介绍

Mouse CD68 (also known as macrosialin) is a type I transmembrane glycoprotein that is highly and specifically expressed by mouse tissue macrophages, and to a lesser extent by dendritic cells. It belongs to the lysosomal/endosomal-associated membrane glycoprotein (LAMP) family and primarily localizes to lysosomes and endosomes with a smaller fraction circulating to the cell surface. CD68 is also a member of the scavenger receptor family. It may play a role in phagocytic activities of tissue macrophages.

实验方案

Product Specific Protocols
IF protocol for CD68 antibody 28058-1-AP	Download protocol
IHC protocol for CD68 antibody 28058-1-AP	Download protocol
WB protocol for CD68 antibody 28058-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

发表文章

Species	Application	Title
pig	IF	Nature Injectable ultrasonic sensor for wireless monitoring of intracranial signals Authors - Hanchuan Tang View Article
	WB,IF	Signal Transduct Target Ther C/EBPβ activation in vascular smooth muscle cells promotes hyperlipidemia-induced phenotypic transition and arterial stiffness Authors - Jun Ma View Article
mouse	IF	Nat Aging Activation of AMPK by GLP-1R agonists mitigates Alzheimer-related phenotypes in transgenic mice Authors - Yun Zhang View Article
mouse	IHC	Crit Care Recombinant ACE2 protein protects against acute lung injury induced by SARS-CoV-2 spike RBD protein. Authors - Lingbing Zhang View Article
mouse	IF	ACS Nano Inflammation and Acinar Cell Dual-Targeting Nanomedicines for Synergistic Treatment of Acute Pancreatitis via Ca2+ Homeostasis Regulation and Pancreas Autodigestion Inhibition Authors - Yanan Wang View Article
mouse	IF	J Pineal Res Extracellular vesicles derived from melatonin-preconditioned mesenchymal stem cells containing USP29 repair traumatic spinal cord injury by stabilizing NRF2. Authors - Wei Liu View Article

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验证数据展示

WB analysis of RAW 264.7 using 28058-1-AP

IHC staining of mouse spleen using 28058-1-AP

IHC staining of mouse spleen using 28058-1-AP

IF Staining of human tonsillitis using 28058-1-AP

IF Staining of human tonsillitis using 28058-1-AP

CD68 Polyclonal antibody

CD68 Polyclonal Antibody for WB, IHC, IF-P, ELISA

经过测试的应用

推荐稀释比

发表文章中的应用

产品信息

背景介绍

实验方案

发表文章

Injectable ultrasonic sensor for wireless monitoring of intracranial signals

C/EBPβ activation in vascular smooth muscle cells promotes hyperlipidemia-induced phenotypic transition and arterial stiffness

Activation of AMPK by GLP-1R agonists mitigates Alzheimer-related phenotypes in transgenic mice

Recombinant ACE2 protein protects against acute lung injury induced by SARS-CoV-2 spike RBD protein.

Inflammation and Acinar Cell Dual-Targeting Nanomedicines for Synergistic Treatment of Acute Pancreatitis via Ca2+ Homeostasis Regulation and Pancreas Autodigestion Inhibition

Extracellular vesicles derived from melatonin-preconditioned mesenchymal stem cells containing USP29 repair traumatic spinal cord injury by stabilizing NRF2.