• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0
  • Featured Product
  • KD/KO Validated

Cathepsin L Monoclonal antibody, PBS Only

Cathepsin L Monoclonal Antibody for WB,Indirect ELISA,IHC

Host / Isotype

Mouse / IgG2b

Reactivity

Human, rat, mouse

Applications

WB,Indirect ELISA,IHC

Conjugate

Unconjugated

CloneNo.

1A12G8

Cat No : 66914-1-PBS

Print datasheet

Synonyms

Cathepsin L, cathepsin L1, CATL, CTSL, CTSL1, FLJ31037, Major excreted protein, MEP

验证数据展示

  • WB analysis of A549 using 66914-1-Ig (same clone as 66914-1-PBS)

    WB analysis of A549 using 66914-1-Ig (same clone as 66914-1-PBS)

    A549 cells were subjected to SDS PAGE followed by western blot with 66914-1-Ig (Cathepsin L antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66914-1-PBS in a different storage buffer formulation.

  • WB analysis of A549 using 66914-1-Ig (same clone as 66914-1-PBS)

    WB analysis of A549 using 66914-1-Ig (same clone as 66914-1-PBS)

    WB result of Cathepsin L antibody (66914-1-Ig; 1:6000; incubated at room temperature for 1.5 hours) with sh-Control and sh-Cathepsin L transfected A549 cells. This data was developed using the same antibody clone with 66914-1-PBS in a different storage buffer formulation.

  • IHC staining of human prostate cancer using 66914-1-Ig (same clone as 66914-1-PBS)

    IHC staining of human prostate cancer using 66914-1-Ig (same clone as 66914-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 66914-1-Ig (Cathepsin L antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66914-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with CTSL1 antibody included
Planning an IHC experiment? We recommend our IHCeasy CTSL1 Ready-To-Use IHC Kit. CTSL1 primary antibody included.

产品信息

66914-1-PBS targets Cathepsin L in WB, IHC, Indirect ELISA applications and shows reactivity with Human, rat, mouse samples.

Tested Applications WB,Indirect ELISA,IHC
Tested Reactivity Human, rat, mouse
Immunogen Cathepsin L fusion protein Ag27951 种属同源性预测
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Full Name cathepsin L1
Synonyms Cathepsin L, cathepsin L1, CATL, CTSL, CTSL1, FLJ31037, Major excreted protein, MEP
Calculated Molecular Weight 38 kDa
Observed Molecular Weight43 kDa
GenBank Accession NumberBC012612
Gene Symbol CTSL1
Gene ID (NCBI) 1514
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP07711
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

背景介绍

CTSL1(Cathepsin L1) is also named as CTSL and MEP, belongs to the peptidase C1 family. It is a lysosomal proteinase whose expression is also up-regulated in the skeletal muscle during starvation(PMID:20088826). It plays an intracellular role in normal intestinal epithelial polarization and initiation of neoplasia(PMID:17622569). CTSL1 also improves cardiac function and inhibits cardiac hypertrophy, inflammation, and fibrosis through blocking AKT/GSK3B signaling(PMID:19096818). The full length protein is 38 kDa with a signal peptide, two propeptide and a glycosylation site. It has been detected the 36 kDa, 39 kDa, 29 kDa and 21 kDa in rat gastrocnemius muscle. These forms of cathepsin L could either be attributed to differences in glycosylation or to partial processing of the proenzyme. (PMID:11696001)