验证数据展示
经过测试的应用
| Positive WB detected in | A549 cells, NCI-H1299 cells, HUVEC cells, HepG2 cells, HeLa cells |
| Positive IHC detected in | human prostate cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HEK-293 cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:8000 |
| Immunohistochemistry (IHC) | IHC : 1:500-1:2000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:400-1:2000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| KD/KO | See 1 publications below |
| WB | See 4 publications below |
| IHC | See 1 publications below |
产品信息
66914-1-Ig targets Cathepsin L in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human samples.
| 经测试应用 | WB, IHC, IF/ICC, ELISA Application Description |
| 文献引用应用 | WB, IHC |
| 经测试反应性 | human |
| 文献引用反应性 | human |
| 免疫原 |
CatNo: Ag27951 Product name: Recombinant human Cathepsin L protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 1-333 aa of BC012612 Sequence: MNPTLILAAFCLGIASATLTFDHSLEAQWTKWKAMHNRLYGMNEEGWRRAVWEKNVKMIELHNQEYREGKHSFTMAMNAFGDMTSEEFRQVMNGFQNRKPRKGKVFQEPLFYEAPRSVDWREKGYVTPVKNQGQCGSCWAFSATGALEGQMFRKTGRLISLSEQNLVDCSGPQGNEGCNGGLMDYAFQYVQDNGGLDSEESYPYEATEESCKYNPKYSVANDTGFVDIPKQEKALMKAVATVGPISVAIDAGHESFLFYKEGIYFEPDCSSEDMDHGVLVVGYGFESTESDNNKYWLVKNSWGEEWGMGGYVKMAKDRRNHCGIASAASYPTV 种属同源性预测 |
| 宿主/亚型 | Mouse / IgG2b |
| 抗体类别 | Monoclonal |
| 产品类型 | Antibody |
| 全称 | cathepsin L1 |
| 别名 | CTSL1, 1A12G8, Cathepsin L heavy chain, Cathepsin L light chain, cathepsin L1 |
| 计算分子量 | 38 kDa |
| 观测分子量 | 43 kDa |
| GenBank蛋白编号 | BC012612 |
| 基因名称 | Cathepsin L |
| Gene ID (NCBI) | 1514 |
| RRID | AB_2882241 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Protein A purification |
| UNIPROT ID | P07711 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
CTSL1(Cathepsin L1) is also named as CTSL and MEP, belongs to the peptidase C1 family. It is a lysosomal proteinase whose expression is also up-regulated in the skeletal muscle during starvation(PMID:20088826). It plays an intracellular role in normal intestinal epithelial polarization and initiation of neoplasia(PMID:17622569). CTSL1 also improves cardiac function and inhibits cardiac hypertrophy, inflammation, and fibrosis through blocking AKT/GSK3B signaling(PMID:19096818). The full length protein is 38 kDa with a signal peptide, two propeptide and a glycosylation site. It has been detected the 36 kDa, 39 kDa, 29 kDa and 21 kDa in rat gastrocnemius muscle. These forms of cathepsin L could either be attributed to differences in glycosylation or to partial processing of the proenzyme. (PMID:11696001)
实验方案
| Product Specific Protocols | |
|---|---|
| IF protocol for Cathepsin L antibody 66914-1-Ig | Download protocol |
| IHC protocol for Cathepsin L antibody 66914-1-Ig | Download protocol |
| WB protocol for Cathepsin L antibody 66914-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Mol Cancer Sulindac (K-80003) with nab-paclitaxel and gemcitabine overcomes drug-resistant pancreatic cancer | ||
Clin Sci (Lond) Loss of FoxO1 activates an alternate mechanism of mitochondrial quality control for healthy adipose browning | ||
Tissue Cell High expression of SERPINE1 and CTSL in keratinocytes in pressure injury caused by ischemia-reperfusion injury | ||
Cancer Sci Methyltransferase-like 3 promotes cervical cancer metastasis by enhancing cathepsin L mRNA stability in an N6-methyladenosine-dependent manner
| ||
Chem Biodivers Design, Synthesis and Biological Evaluation of Naphthalimide-Polyamine Conjugate as a Potential Anti-Colorectal Cancer Agent |
