• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0

验证数据展示

  • WB analysis using 66534-1-Ig (same clone as 66534-1-PBS)

    WB analysis using 66534-1-Ig (same clone as 66534-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 66534-1-Ig (Cathepsin D antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66534-1-PBS in a different storage buffer formulation.

  • IHC staining of human liver cancer using 66534-1-Ig (same clone as 66534-1-PBS)

    IHC staining of human liver cancer using 66534-1-Ig (same clone as 66534-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 66534-1-Ig (Cathepsin D antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66534-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with Cathepsin D antibody included
  • IHC staining of human liver cancer using 66534-1-Ig (same clone as 66534-1-PBS)

    IHC staining of human liver cancer using 66534-1-Ig (same clone as 66534-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 66534-1-Ig (Cathepsin D antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66534-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with Cathepsin D antibody included
  • IF Staining of HepG2 using 66534-1-Ig (same clone as 66534-1-PBS)

    IF Staining of HepG2 using 66534-1-Ig (same clone as 66534-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using Cathepsin D antibody (66534-1-Ig, Clone: 2F6F7 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66534-1-PBS in a different storage buffer formulation.

  • FC experiment of HepG2 using 66534-1-Ig (same clone as 66534-1-PBS)

    FC experiment of HepG2 using 66534-1-Ig (same clone as 66534-1-PBS)

    1X10^6 HepG2 cells were intracellularly stained with 0.4 ug Anti-Human Cathepsin D (66534-1-Ig, Clone:2F6F7) and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Mouse IgG2b Isotype Control (MPC-11) (65128-1-Ig, Clone: MPC-11) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 66534-1-PBS in a different storage buffer formulation.

  • IHC staining of human liver cancer using 66534-1-Ig (same clone as 66534-1-PBS)

    IHC staining of human liver cancer using 66534-1-Ig (same clone as 66534-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 66534-1-Ig (Cathepsin D antibody) at dilution of 1:1000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66534-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with Cathepsin D antibody included
  • Featured Product
  • KD/KO验证

Cathepsin D Monoclonal antibody, PBS Only

Cathepsin D Monoclonal Antibody for WB, IHC, IF/ICC, FC (Intra), ELISA
Cat No. 66534-1-PBS

产品说明书

CloneNo. 2F6F7

宿主/亚型

Mouse / IgG2b

种属反应性

human

应用

WB, IHC, IF/ICC, FC (Intra), ELISA

别名

CTSD, CPSD, CLN10, Cathepsin D light chain, Cathepsin D heavy chain

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

点击咨询售前客服

点击咨询售后客服

Planning an IHC experiment? We recommend our IHCeasy Cathepsin D Ready-To-Use IHC Kit. Cathepsin D primary antibody included.

产品信息

66534-1-PBS targets Cathepsin D in WB, IHC, IF/ICC, FC (Intra), ELISA applications and shows reactivity with human samples.

经测试应用 WB, IHC, IF/ICC, FC (Intra), ELISA Application Description
经测试反应性 human
免疫原 Cathepsin D fusion protein Ag15254 种属同源性预测
宿主/亚型 Mouse / IgG2b
抗体类别 Monoclonal
产品类型 Antibody
全称 cathepsin D
别名 CTSD, CPSD, CLN10, Cathepsin D light chain, Cathepsin D heavy chain
计算分子量 412 aa, 45 kDa
观测分子量 32 kDa, 48 kDa, 52 kDa
GenBank蛋白编号BC016320
基因名称 Cathepsin D
Gene ID (NCBI) 1509
RRIDAB_2881897
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDP07339
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

CTSD (Cathepsin D) also named CPSD, belongs to the peptidase A1 family. It is ubiquitously expressed and is involved in proteolytic degradation, cell invasion, and apoptosis. Human CTSD is synthesized as a 52-kDa precursor that is converted into an active 48-kDa single-chain intermediate in the endosomes, and then into a fully active mature form, composed of a 34-kDa heavy chain and a 14-kDa light chain, in the lysosomes. It is a lysosomal acid protease found in neutrophils and monocytes and involved in the pathogenesis of several diseases such as breast cancer and possibly Alzheimer disease (PMID: 27114232, PMID: 30717773, PMID: 30051532).