验证数据展示
经过测试的应用
| Positive WB detected in | Saos-2 cells, MCF-7 cells, T-47D cells, HepG2 cells |
| Positive IHC detected in | human liver cancer tissue, human breast cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HepG2 cells |
| Positive FC (Intra) detected in | HepG2 cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| Immunohistochemistry (IHC) | IHC : 1:500-1:2000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
66534-1-Ig targets Cathepsin D in WB, IHC, IF/ICC, FC (Intra), ELISA applications and shows reactivity with human samples.
| 经测试应用 | WB, IHC, IF/ICC, FC (Intra), ELISA Application Description |
| 文献引用应用 | WB, IHC, IF |
| 经测试反应性 | human |
| 文献引用反应性 | human, rat |
| 免疫原 | Cathepsin D fusion protein Ag15254 种属同源性预测 |
| 宿主/亚型 | Mouse / IgG2b |
| 抗体类别 | Monoclonal |
| 产品类型 | Antibody |
| 全称 | cathepsin D |
| 别名 | CTSD, CPSD, CLN10, Cathepsin D light chain, Cathepsin D heavy chain |
| 计算分子量 | 412 aa, 45 kDa |
| 观测分子量 | 32 kDa, 48 kDa, 52 kDa |
| GenBank蛋白编号 | BC016320 |
| 基因名称 | Cathepsin D |
| Gene ID (NCBI) | 1509 |
| RRID | AB_2881897 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Protein A purification |
| UNIPROT ID | P07339 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
CTSD (Cathepsin D) also named CPSD, belongs to the peptidase A1 family. It is ubiquitously expressed and is involved in proteolytic degradation, cell invasion, and apoptosis. Human CTSD is synthesized as a 52-kDa precursor that is converted into an active 48-kDa single-chain intermediate in the endosomes, and then into a fully active mature form, composed of a 34-kDa heavy chain and a 14-kDa light chain, in the lysosomes. It is a lysosomal acid protease found in neutrophils and monocytes and involved in the pathogenesis of several diseases such as breast cancer and possibly Alzheimer disease (PMID: 27114232, PMID: 30717773, PMID: 30051532).
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for Cathepsin D antibody 66534-1-Ig | Download protocol |
| IHC protocol for Cathepsin D antibody 66534-1-Ig | Download protocol |
| IF protocol for Cathepsin D antibody 66534-1-Ig | Download protocol |
| FC protocol for Cathepsin D antibody 66534-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Gastroenterology Proteomic characterization identifies clinically relevant subgroups of gastrointestinal stromal tumors | ||
Life (Basel) Melibiose Confers a Neuroprotection against Cerebral Ischemia/Reperfusion Injury by Ameliorating Autophagy Flux via Facilitation of TFEB Nuclear Translocation in Neurons. | ||
Cell Rep Intracellular complement C5a/C5aR1 stabilizes β-catenin to promote colorectal tumorigenesis.
| ||
FEBS Lett Senile plaques in Alzheimer's disease arise from Aβ- and Cathepsin D-enriched mixtures leaking out during intravascular haemolysis and microaneurysm rupture | ||
Ecotoxicol Environ Saf The inhibition of TRPML1/TFEB leads to lysosomal biogenesis disorder, contributes to developmental fluoride neurotoxicity | ||
Ecotoxicol Environ Saf TFE3-mediated impairment of lysosomal biogenesis and defective autophagy contribute to fluoride-induced hepatotoxicity |
