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验证数据展示

  • WB analysis using 85841-5-RR (same clone as 85841-5-PBS)

    WB analysis using 85841-5-RR (same clone as 85841-5-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 85841-5-RR (Cathepsin B antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 85841-5-PBS in a different storage buffer formulation.

  • IHC staining of mouse brain using 85841-5-RR (same clone as 85841-5-PBS)

    IHC staining of mouse brain using 85841-5-RR (same clone as 85841-5-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 85841-5-RR (Cathepsin B antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 85841-5-PBS in a different storage buffer formulation.

  • IHC staining of mouse brain using 85841-5-RR (same clone as 85841-5-PBS)

    IHC staining of mouse brain using 85841-5-RR (same clone as 85841-5-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 85841-5-RR (Cathepsin B antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 85841-5-PBS in a different storage buffer formulation.

  • IF Staining of RAW 264.7 using 85841-5-RR (same clone as 85841-5-PBS)

    IF Staining of RAW 264.7 using 85841-5-RR (same clone as 85841-5-PBS)

    Immunofluorescent analysis of (4% PFA) fixed RAW 264.7 cells using Cathepsin B antibody (85841-5-RR, Clone: 243020A5 ) at dilution of 1:1000 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 85841-5-PBS in a different storage buffer formulation.

  • FC experiment of RAW 264.7 using 85841-5-RR (same clone as 85841-5-PBS)

    FC experiment of RAW 264.7 using 85841-5-RR (same clone as 85841-5-PBS)

    1x10^6 RAW 264.7 cells were intracellularly stained with 0.25 ug Cathepsin B Recombinant antibody (85841-5-RR, Clone:243020A5) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug Rabbit IgG Isotype Control RecAb (98136-1-RR, Clone: 240953C9) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer. This data was developed using the same antibody clone with 85841-5-PBS in a different storage buffer formulation.

Mouse Cathepsin B Recombinant monoclonal antibody, PBS Only

Cathepsin B Uni-rAb® Recombinant Antibody for WB, IHC, IF/ICC, FC (Intra), Indirect ELISA
Cat No. 85841-5-PBS

产品说明书

CloneNo. 243020A5

宿主/亚型

Rabbit / IgG

种属反应性

mouse

应用

WB, IHC, IF/ICC, FC (Intra), Indirect ELISA

别名

Cathepsin B heavy chain, Cathepsin B light chain, Cathepsin B1, Ctsb, EC:3.4.22.1

缓冲液配方:  PBS Only
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
规格价格库存

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产品信息

85841-5-PBS targets Cathepsin B in WB, IHC, IF/ICC, FC (Intra), Indirect ELISA applications and shows reactivity with mouse samples.

经测试应用 WB, IHC, IF/ICC, FC (Intra), Indirect ELISA Application Description
经测试反应性 mouse
免疫原

CatNo: Eg3028

Product name: Recombinant Mouse Ctsb protein (rFc Tag)

Source: mammalian cells-derived, V37

Tag: C-rFc

Domain: 18-339 aa of NM_007798.3

Sequence: HDKPSFHPLSDDLINYINKQNTTWQAGRNFYNVDISYLKKLCGTVLGGPKLPGRVAFGEDIDLPETFDAREQWSNCPTIGQIRDQGSCGSCWAFGAVEAISDRTCIHTNGRVNVEVSAEDLLTCCGIQCGDGCNGGYPSGAWSFWTKKGLVSGGVYNSHVGCLPYTIPPCEHHVNGSRPPCTGEGDTPRCNKSCEAGYSPSYKEDKHFGYTSYSVSNSVKEIMAEIYKNGPVEGAFTVFSDFLTYKSGVYKHEAGDMMGGHAIRILGWGVENGVPYWLAANSWNLDWGDNGFFKILRGENHCGIESEIVAGIPRTDQYWGRF

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 cathepsin B
别名 Cathepsin B heavy chain, Cathepsin B light chain, Cathepsin B1, Ctsb, EC:3.4.22.1
计算分子量37 kDa
观测分子量31 kDa, 39 kDa
GenBank蛋白编号NM_007798.3
基因名称 Ctsb
Gene ID (NCBI) 13030
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purification
UNIPROT IDP10605
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

CTSB(Cathepsin B) is also named as CPSB and belongs to the peptidase C1 family. It participates in intracellular degradation and turnover of proteins. Cathepsin B precursors found in human malignant ascites fluid do not possess mannose-rich carbohydrates suggesting that a defect in the post translational processing of carbohydrate moieties on tumor. Cathepsin B exists as both glycosylated and unglycosylated forms(PMID:1637335). In rat macrophages and hepatocytes pro- cathepsin B is 39 kDa (unglycosylated =35 kDa), whereas in human fibroblasts procathepsin B is 44.5-46kDa (unglycosylated=39kDa)(PMID:2097084). It can be detected the 39 kDa form of pro-CTSB, 31 kDa mature forms in mouse brain by western blot.

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