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验证数据展示

  • FC experiment of HeLa using CL488-67956

    FC experiment of HeLa using CL488-67956

    1X10^6 HeLa cells were intracellularly stained with 0.4 ug CoraLite® Plus 488 Anti-Human Caspase 7 (CL488-67956, Clone:3C9H4) (red), or 0.4 ug Mouse IgG2a Isotype Control (CL488-66360-2, Clone: K11A1B2A2) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

CoraLite® Plus 488-conjugated Caspase 7 Monoclonal antibody

Caspase 7 Monoclonal Antibody for FC (Intra)
Cat No. CL488-67956

产品说明书

CloneNo. 3C9H4

宿主/亚型

Mouse / IgG2a

种属反应性

human, mouse

应用

FC (Intra)

别名

CASP7, CASP-7, CASP 7, Apoptotic protease Mch-3, Apoptotic protease Mch 3

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  CoraLite® Plus 488
规格: 

-/ -

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经过测试的应用

Positive FC (Intra) detected inHeLa cells
Planning an IHC experiment? We recommend our IHCeasy CASP7 Ready-To-Use IHC Kit. CASP7 primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 67956-1-Ig

推荐稀释比

应用推荐稀释比
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-67956 targets Caspase 7 in FC (Intra) applications and shows reactivity with human, mouse samples.

经测试应用 FC (Intra) Application Description
经测试反应性 human, mouse
免疫原 Caspase 7 fusion protein Ag27601 种属同源性预测
宿主/亚型 Mouse / IgG2a
抗体类别 Monoclonal
产品类型 Antibody
全称 caspase 7, apoptosis-related cysteine peptidase
别名 CASP7, CASP-7, CASP 7, Apoptotic protease Mch-3, Apoptotic protease Mch 3
计算分子量 303 aa, 34 kDa
观测分子量 35 kDa
GenBank蛋白编号BC015799
基因名称 Caspase 7
Gene ID (NCBI) 840
RRIDAB_2934598
偶联类型 CoraLite® Plus 488 Fluorescent Dye
最大激发/发射波长493 nm / 522 nm
形式 Liquid
纯化方式Protein A purification
UNIPROT IDP55210
储存缓冲液 PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA , pH 7.3
储存条件Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Caspase 7(CASP7), like caspases 3 and 6, contains a short prodomain and, upon apoptotic induction, the 35 kDa proform is converted into a 32 kDa intermediate or preactive form which is further processed into two active subunits consisting of the p20 or large (18 kDa) subunit and the p10 or small (11 kDa) subunit and it is present in the brain, which is up-regulated and activated after traumatic injury(PMID:15953353). Caspase-7 is classified as a member of the subgroup of cysteine proteases most related to the Caenorhabditis elegans factor CED-3, which also includes caspase-3, -6, and -9(PMID:9426061). The protein is involved in the activation cascade of caspases responsible for apoptosis execution.

实验方案

Product Specific Protocols
FC protocol for CL Plus 488 Caspase 7 antibody CL488-67956Download protocol
Standard Protocols
Click here to view our Standard Protocols