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验证数据展示

  • WB analysis of SGC-7901 using 67398-1-Ig (same clone as 67398-1-PBS)

    WB analysis of SGC-7901 using 67398-1-Ig (same clone as 67398-1-PBS)

    SGC-7901 cells were subjected to SDS PAGE followed by western blot with 67398-1-Ig (Caspase 4 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67398-1-PBS in a different storage buffer formulation.

  • WB analysis of HL-60 using 67398-1-Ig (same clone as 67398-1-PBS)

    WB analysis of HL-60 using 67398-1-Ig (same clone as 67398-1-PBS)

    HL-60 cells were subjected to SDS PAGE followed by western blot with 67398-1-Ig (Caspase 4 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67398-1-PBS in a different storage buffer formulation.

  • IF Staining of human liver cancer using 67398-1-Ig (same clone as 67398-1-PBS)

    IF Staining of human liver cancer using 67398-1-Ig (same clone as 67398-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed human liver cancer tissue using Caspase 4 antibody (67398-1-Ig, Clone: 1D1E12 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67398-1-PBS in a different storage buffer formulation.

  • IHC staining of human liver cancer using 67398-1-Ig (same clone as 67398-1-PBS)

    IHC staining of human liver cancer using 67398-1-Ig (same clone as 67398-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 67398-1-Ig (Caspase 4 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67398-1-PBS in a different storage buffer formulation.

  • IHC staining of human liver cancer using 67398-1-Ig (same clone as 67398-1-PBS)

    IHC staining of human liver cancer using 67398-1-Ig (same clone as 67398-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 67398-1-Ig (Caspase 4 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67398-1-PBS in a different storage buffer formulation.

  • IF Staining of HeLa using 67398-1-Ig (same clone as 67398-1-PBS)

    IF Staining of HeLa using 67398-1-Ig (same clone as 67398-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using Caspase 4 antibody (67398-1-Ig, Clone: 1D1E12 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1). This data was developed using the same antibody clone with 67398-1-PBS in a different storage buffer formulation.

Caspase 4 Monoclonal antibody, PBS Only

Caspase 4 Monoclonal Antibody for WB, IHC, IF/ICC, IF-P, Indirect ELISA
Cat No. 67398-1-PBS

产品说明书

CloneNo. 1D1E12

宿主/亚型

Mouse / IgG1

种属反应性

human

应用

WB, IHC, IF/ICC, IF-P, Indirect ELISA

别名

CASP4, Caspase4, 1D1E12, CASP 4, CASP-4

缓冲液配方:  PBS Only
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
规格价格库存

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产品信息

67398-1-PBS targets Caspase 4 in WB, IHC, IF/ICC, IF-P, Indirect ELISA applications and shows reactivity with human samples.

经测试应用 WB, IHC, IF/ICC, IF-P, Indirect ELISA Application Description
经测试反应性 human
免疫原

CatNo: Ag29488

Product name: Recombinant human Caspase 4 protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 1-377 aa of BC017839

Sequence: MAEGNHRKKPLKVLESLGKDFLTGVLDNLVEQNVLNWKEEEKKKYYDAKTEDKVRVMADSMQEKQRMAGQMLLQTFFNIDQISPNKKAHPNMEAGPPESGESTDALKLCPHEEFLRLCKERAEEIYPIKERNNRTRLALIICNTEFDHLPPRNGADFDITGMKELLEGLDYSVDVEENLTARDMESALRAFATRPEHKSSDSTFLVLMSHGILEGICGTVHDEKKPDVLLYDTIFQIFNNRNCLSLKDKPKVIIVQACRGANRGELWVRDSPASLEVASSQSSENLEEDAVYKTHVEKDFIAFCSSTPHNVSWRDSTMGSIFITQLITCFQKYSWCCHLEEVFRKVQQSFETPRAKAQMPTIERLSMTRYFYLFPGN

种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 caspase 4, apoptosis-related cysteine peptidase
别名 CASP4, Caspase4, 1D1E12, CASP 4, CASP-4
计算分子量 377 aa, 43 kDa
观测分子量 43-48 kDa
GenBank蛋白编号BC017839
基因名称 Caspase 4
Gene ID (NCBI) 837
RRIDAB_2882641
偶联类型 Unconjugated
形式Liquid
纯化方式Protein G purification
UNIPROT IDP49662
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Caspase 4 (CASP4), is a member of the cysteine-aspartic acid protease (caspase) family. Sequential activation of caspases plays a central role in the execution-phase of cell apoptosis.Caspase 4 is able to cleave and activate its own precursor protein, as well as caspase 1 precursor. Overexpression of caspase 4 will induce cell apoptosis.

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