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验证数据展示

  • WB analysis using 85196-3-RR (same clone as 85196-3-PBS)

    WB analysis using 85196-3-RR (same clone as 85196-3-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 85196-3-RR (Caspase 10 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 85196-3-PBS in a different storage buffer formulation.

  • WB analysis using 85196-3-RR (same clone as 85196-3-PBS)

    WB analysis using 85196-3-RR (same clone as 85196-3-PBS)

    Staurosporine treated Jurkat cells were subjected to SDS PAGE followed by western blot with 85196-3-RR (Caspase 10 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 85196-3-PBS in a different storage buffer formulation.

  • IHC staining of Jurkat using 85196-3-RR (same clone as 85196-3-PBS)

    IHC staining of Jurkat using 85196-3-RR (same clone as 85196-3-PBS)

    Immunohistochemical analysis of paraffin-embedded Jurkat cells slide using 85196-3-RR (Caspase 10 antibody) at dilution of 1:1000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 85196-3-PBS in a different storage buffer formulation.

  • IF Staining of Jurkat using 85196-3-RR (same clone as 85196-3-PBS)

    IF Staining of Jurkat using 85196-3-RR (same clone as 85196-3-PBS)

    Immunofluorescent analysis of (4% PFA) fixed Jurkat cells using Caspase 10 antibody (85196-3-RR, Clone: 242353H6 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 85196-3-PBS in a different storage buffer formulation.

  • FC experiment of HepG2 using 85196-3-RR (same clone as 85196-3-PBS)

    FC experiment of HepG2 using 85196-3-RR (same clone as 85196-3-PBS)

    1x10^6 HepG2 cells were intracellularly stained with 0.25 ug Caspase 10 Recombinant Antibody (85196-3-RR, Clone:242353H6) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Rabbit IgG Isotype Control RecAb (98136-1-RR, Clone: 240953C9) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer. This data was developed using the same antibody clone with 85196-3-PBS in a different storage buffer formulation.

Caspase 10 Recombinant monoclonal antibody, PBS Only

Caspase 10 Uni-rAb® Recombinant Antibody for WB, IHC, IF/ICC, FC (Intra), Indirect ELISA
Cat No. 85196-3-PBS

产品说明书

CloneNo. 242353H6

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IHC, IF/ICC, FC (Intra), Indirect ELISA

别名

CASP10, Caspase10, 242353H6, Apoptotic protease Mch 4, Apoptotic protease Mch-4

缓冲液配方:  PBS Only
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
规格价格库存

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产品信息

85196-3-PBS targets Caspase 10 in WB, IHC, IF/ICC, FC (Intra), Indirect ELISA applications and shows reactivity with human samples.

经测试应用 WB, IHC, IF/ICC, FC (Intra), Indirect ELISA Application Description
经测试反应性 human
免疫原

Peptide

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 caspase 10, apoptosis-related cysteine peptidase
别名 CASP10, Caspase10, 242353H6, Apoptotic protease Mch 4, Apoptotic protease Mch-4
计算分子量 59 kDa
观测分子量59 kDa
GenBank蛋白编号BC042844
基因名称 Caspase 10
Gene ID (NCBI) 843
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purfication
UNIPROT IDQ92851
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

CASP10(Caspase-10) is also named as MCH4 and belongs to the peptidase C14A family. It is recruited to the CD99 and p55 tumor necrosis factor receptor signaling complexes and promoting CD95 (APO1/Fas) apoptosis. It may also play a critical role in PTK7-knockdown-induced apoptosis, downstream of mitochondria(PMID:21103379). This protein can be Cleavage by granzyme B and autocatalytic activity generate two active subunits. Defects in CASP10 are the cause of autoimmune lymphoproliferative syndrome type 2A (ALPS2A)(PMID:16446975), familial non-Hodgkin lymphoma (NHL)(PMID:12010812) and gastric cancer (GASC)(PMID:11973654). The full length has a propeptide with 219 amino acid and 6 isoforms produced by alternative splicing.

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