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验证数据展示

  • WB analysis using 86977-4-RR (same clone as 86977-4-PBS)

    WB analysis using 86977-4-RR (same clone as 86977-4-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 86977-4-RR (Calponin antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 86977-4-PBS in a different storage buffer formulation.

  • Sandwich ELISA sample test of MP02782-1

    Sandwich ELISA sample test of MP02782-1

    The mean Calponin concentration was determined to be 26.4 ng/mL in mouse lung tissue extract based on a 4.8 mg/mL extract load.

  • Sandwich ELISA standard curve of MP02782-1

    Sandwich ELISA standard curve of MP02782-1

    Sandwich ELISA standard curve of MP02782-1, Human Calponin Recombinant Matched Antibody Pair - PBS only. 86977-5-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard Ag21384. 86977-4-PBS was HRP conjugated as the detection antibody. Range: 78.1-5000 pg/mL.

  • IHC staining of human appendix using 86977-4-RR (same clone as 86977-4-PBS)

    IHC staining of human appendix using 86977-4-RR (same clone as 86977-4-PBS)

    Immunohistochemical analysis of paraffin-embedded human appendix tissue slide using 86977-4-RR (Calponin antibody) at dilution of 1:2000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 86977-4-PBS in a different storage buffer formulation.

  • IHC staining of human appendix using 86977-4-RR (same clone as 86977-4-PBS)

    IHC staining of human appendix using 86977-4-RR (same clone as 86977-4-PBS)

    Immunohistochemical analysis of paraffin-embedded human appendix tissue slide using 86977-4-RR (Calponin antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 86977-4-PBS in a different storage buffer formulation.

  • IF Staining of mouse heart using 86977-4-RR (same clone as 86977-4-PBS)

    IF Staining of mouse heart using 86977-4-RR (same clone as 86977-4-PBS)

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse heart tissue using Calponin antibody (86977-4-RR, Clone: 252040C7 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 86977-4-PBS in a different storage buffer formulation.

  • IF Staining of mouse heart using 86977-4-RR (same clone as 86977-4-PBS)

    IF Staining of mouse heart using 86977-4-RR (same clone as 86977-4-PBS)

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse heart tissue using Calponin antibody (86977-4-RR, Clone: 252040C7 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 86977-4-PBS in a different storage buffer formulation.

  • Affinity of 86977-4-RR

    Affinity of 86977-4-RR

    Biolayer interferometry (BLl) kinetic assays of 86977-4-RR against Human Calponin were performed. The affinity constant is 1.34 nM.

Calponin Recombinant monoclonal antibody, PBS Only (Detector)

Calponin Uni-rAb® Recombinant Antibody for WB, IHC, IF-P, Sandwich ELISA, Indirect ELISA
Cat No. 86977-4-PBS

产品说明书

CloneNo. 252040C7

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, IF-P, Sandwich ELISA, Indirect ELISA

别名

CNN1, Basic calponin, Calponin 1, Calponin-1, CNN 1

缓冲液配方:  PBS Only
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
规格价格库存

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产品信息

86977-4-PBS targets Calponin as part of a matched antibody pair:

MP02782-1: 86977-5-PBS capture and 86977-4-PBS detection (validated in Sandwich ELISA)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

经测试应用 WB, IHC, IF-P, Sandwich ELISA, Indirect ELISA Application Description
经测试反应性 human, mouse, rat
免疫原

CatNo: Ag21384

Product name: Recombinant human Calponin protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 225-265 aa of BC022015

Sequence: KRQIFEPGLGMEHCDTLNVSLQMGSNKGASQRGMTVYGLPR

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 calponin 1, basic, smooth muscle
别名 CNN1, Basic calponin, Calponin 1, Calponin-1, CNN 1
计算分子量 33 kDa
观测分子量 34 kDa
GenBank蛋白编号BC022015
基因名称 Calponin
Gene ID (NCBI) 1264
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purification
UNIPROT IDP51911
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Calponin is a family of actin filament-associated proteins which regulate smooth muscle cell contraction. Three isoforms of calponin exist: calponin h1 (CNN1) , calponin h2 (CNN2) and calponin 3 (CNN3). CNN1 is a basic 34-kDa protein specifically expressed in smooth muscle and a marker of smooth muscle cell differentiation.

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