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验证数据展示

  • WB analysis using 86977-4-RR

    WB analysis using 86977-4-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 86977-4-RR (Calponin antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • IHC staining of human appendix using 86977-4-RR

    IHC staining of human appendix using 86977-4-RR

    Immunohistochemical analysis of paraffin-embedded human appendix tissue slide using 86977-4-RR (Calponin antibody) at dilution of 1:2000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human appendix using 86977-4-RR

    IHC staining of human appendix using 86977-4-RR

    Immunohistochemical analysis of paraffin-embedded human appendix tissue slide using 86977-4-RR (Calponin antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of mouse heart using 86977-4-RR

    IF Staining of mouse heart using 86977-4-RR

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse heart tissue using Calponin antibody (86977-4-RR, Clone: 252040C7 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of mouse heart using 86977-4-RR

    IF Staining of mouse heart using 86977-4-RR

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse heart tissue using Calponin antibody (86977-4-RR, Clone: 252040C7 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Affinity of 86977-4-RR

    Affinity of 86977-4-RR

    Biolayer interferometry (BLl) kinetic assays of 86977-4-RR against Human Calponin were performed. The affinity constant is 1.34 nM.

Calponin Recombinant monoclonal antibody

Calponin Uni-rAb® Recombinant Antibody for WB, IHC, IF-P, ELISA
Cat No. 86977-4-RR

产品说明书

CloneNo. 252040C7

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, IF-P, ELISA

别名

CNN1, Basic calponin, Calponin 1, Calponin-1, CNN 1

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inmouse colon tissue, mouse bladder tissue, rat bladder tissue
Positive IHC detected inhuman appendix tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF-P detected inmouse heart tissue

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:2000-1:10000
Immunohistochemistry (IHC)IHC : 1:1000-1:4000
Immunofluorescence (IF)-PIF-P : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

86977-4-RR targets Calponin in WB, IHC, IF-P, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IF-P, ELISA Application Description
经测试反应性 human, mouse, rat
免疫原

CatNo: Ag21384

Product name: Recombinant human Calponin protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 225-265 aa of BC022015

Sequence: KRQIFEPGLGMEHCDTLNVSLQMGSNKGASQRGMTVYGLPR

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 calponin 1, basic, smooth muscle
别名 CNN1, Basic calponin, Calponin 1, Calponin-1, CNN 1
计算分子量 33 kDa
观测分子量 34 kDa
GenBank蛋白编号BC022015
基因名称 Calponin
Gene ID (NCBI) 1264
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purification
UNIPROT IDP51911
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Calponin is a family of actin filament-associated proteins which regulate smooth muscle cell contraction. Three isoforms of calponin exist: calponin h1 (CNN1) , calponin h2 (CNN2) and calponin 3 (CNN3). CNN1 is a basic 34-kDa protein specifically expressed in smooth muscle and a marker of smooth muscle cell differentiation.

实验方案

Product Specific Protocols
IF protocol for Calponin antibody 86977-4-RRDownload protocol
IHC protocol for Calponin antibody 86977-4-RRDownload protocol
WB protocol for Calponin antibody 86977-4-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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