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验证数据展示

  • WB analysis using 13938-1-AP (same clone as 13938-1-PBS)

    WB analysis using 13938-1-AP (same clone as 13938-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 13938-1-AP (Calponin antibody) at dilution of 1:30000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 13938-1-PBS in a different storage buffer formulation.

  • WB analysis of mouse heart using 13938-1-AP (same clone as 13938-1-PBS)

    WB analysis of mouse heart using 13938-1-AP (same clone as 13938-1-PBS)

    mouse heart tissue were subjected to SDS PAGE followed by western blot with 13938-1-AP (CNN1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 13938-1-PBS in a different storage buffer formulation.

  • IF Staining of mouse heart using 13938-1-AP (same clone as 13938-1-PBS)

    IF Staining of mouse heart using 13938-1-AP (same clone as 13938-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse heart tissue using Calponin antibody (13938-1-AP) at dilution of 1:200 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 13938-1-PBS in a different storage buffer formulation.

  • IF Staining of C2C12 using 13938-1-AP (same clone as 13938-1-PBS)

    IF Staining of C2C12 using 13938-1-AP (same clone as 13938-1-PBS)

    Immunofluorescent analysis of (-20°C Methanol) fixed C2C12 cells using Calponin antibody (13938-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 13938-1-PBS in a different storage buffer formulation.

  • IP experiment of mouse lung using 13938-1-AP (same clone as 13938-1-PBS)

    IP experiment of mouse lung using 13938-1-AP (same clone as 13938-1-PBS)

    IP result of anti-Calponin (IP:13938-1-AP, 4ug; Detection:13938-1-AP 1:1000) with mouse lung tissue lysate 2800ug. This data was developed using the same antibody clone with 13938-1-PBS in a different storage buffer formulation.

  • IHC staining of human appendicitis using 13938-1-AP (same clone as 13938-1-PBS)

    IHC staining of human appendicitis using 13938-1-AP (same clone as 13938-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human appendicitis tissue slide using 13938-1-AP (Calponin antibody) at dilution of 1:4000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 13938-1-PBS in a different storage buffer formulation.

  • IF Staining of H9C2 using 13938-1-AP (same clone as 13938-1-PBS)

    IF Staining of H9C2 using 13938-1-AP (same clone as 13938-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed H9C2 cells using Calponin antibody (13938-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 13938-1-PBS in a different storage buffer formulation.

  • IF Staining of C2C12 using 13938-1-AP (same clone as 13938-1-PBS)

    IF Staining of C2C12 using 13938-1-AP (same clone as 13938-1-PBS)

    Immunofluorescent analysis of (-20°C Methanol) fixed C2C12 cells using Calponin antibody (13938-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 13938-1-PBS in a different storage buffer formulation.

  • FC experiment of C2C12 using 13938-1-AP (same clone as 13938-1-PBS)

    FC experiment of C2C12 using 13938-1-AP (same clone as 13938-1-PBS)

    1X10^6 C2C12 cells were intracellularly stained with 0.4 ug Anti-Human Calponin (13938-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer. This data was developed using the same antibody clone with 13938-1-PBS in a different storage buffer formulation.

  • Featured Product
  • KD/KO Validated

Calponin Polyclonal antibody, PBS Only

Calponin Polyclonal Antibody for WB, IHC, IF/ICC, IF-P, FC (Intra), IP, Indirect ELISA
Cat No. 13938-1-PBS

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, IF/ICC, IF-P, FC (Intra), IP, Indirect ELISA

别名

CNN1, Basic calponin, Calponin 1, Calponin-1, CNN 1

缓冲液配方: 
PBS, Azide, Glycerol
偶联物:  Unconjugated
规格价格库存

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产品信息

13938-1-PBS targets Calponin in WB, IHC, IF/ICC, IF-P, FC (Intra), IP, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IF/ICC, IF-P, FC (Intra), IP, Indirect ELISA Application Description
经测试反应性 human, mouse, rat
免疫原

CatNo: Ag4989

Product name: Recombinant human Calponin protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-297 aa of BC022015

Sequence: MSSAHFNRGPAYGLSAEVKNKLAQKYDHQREQELREWIEGVTGRRIGNNFMDGLKDGIILCEFINKLQPGSVKKINESTQNWHQLENIGNFIKAITKYGVKPHDIFEANDLFENTNHTQVQSTLLALASMAKTKGNKVNVGVKYAEKQERKFEPGKLREGRNIIGLQMGTNKFASQQGMTAYGTRRHLYDPKLGTDQPLDQATISLQMGTNKGASQAGMTAPGTKRQIFEPGLGMEHCDTLNVSLQMGSNKGASQRGMTVYGLPRQVYDPKYCLTPEYPELGEPAHNHHAHNYYNSA

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 calponin 1, basic, smooth muscle
别名 CNN1, Basic calponin, Calponin 1, Calponin-1, CNN 1
计算分子量 33 kDa
观测分子量 35 kDa
GenBank蛋白编号BC022015
基因名称 Calponin
Gene ID (NCBI) 1264
RRIDAB_2082010
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDP51911
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Calponin is a family of actin filament-associated proteins which regulate smooth muscle cell contraction. Three isoforms of calponin exist: calponin h1 (CNN1) , calponin h2 (CNN2) and calponin 3 (CNN3). CNN1 is a basic 34-kD protein specifically expressed in smooth muscle and a marker of smooth muscle cell differentiation. This antibody raised against the full-length of human CNN1 may cross-react with CNN2 and CNN3.

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