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验证数据展示

  • WB analysis of HeLa using 12250-1-AP (same clone as 12250-1-PBS)

    WB analysis of HeLa using 12250-1-AP (same clone as 12250-1-PBS)

    WB result of Calpastatin antibody (12250-1-AP; 1:120000; incubated at room temperature for 1.5 hours) with sh-Control and sh-Calpastatin transfected HeLa cells. This data was developed using the same antibody clone with 12250-1-PBS in a different storage buffer formulation.

  • WB analysis using 12250-1-AP (same clone as 12250-1-PBS)

    WB analysis using 12250-1-AP (same clone as 12250-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 12250-1-AP (Calpastatin antibody) at dilution of 1:7000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12250-1-PBS in a different storage buffer formulation.

  • IHC staining of human lung cancer using 12250-1-AP (same clone as 12250-1-PBS)

    IHC staining of human lung cancer using 12250-1-AP (same clone as 12250-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 12250-1-AP (Calpastatin antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 12250-1-PBS in a different storage buffer formulation.

  • IHC staining of human lung cancer using 12250-1-AP (same clone as 12250-1-PBS)

    IHC staining of human lung cancer using 12250-1-AP (same clone as 12250-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 12250-1-AP (Calpastatin antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 12250-1-PBS in a different storage buffer formulation.

  • IP experiment of K-562 using 12250-1-AP (same clone as 12250-1-PBS)

    IP experiment of K-562 using 12250-1-AP (same clone as 12250-1-PBS)

    IP result of anti-Calpastatin (IP:12250-1-AP, 4ug; Detection:12250-1-AP 1:1000) with K-562 cells lysate 4000ug. This data was developed using the same antibody clone with 12250-1-PBS in a different storage buffer formulation.

  • IF Staining of HeLa using 12250-1-AP (same clone as 12250-1-PBS)

    IF Staining of HeLa using 12250-1-AP (same clone as 12250-1-PBS)

    Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using Calpastatin antibody (12250-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 12250-1-PBS in a different storage buffer formulation.

  • FC experiment of HeLa using 12250-1-AP (same clone as 12250-1-PBS)

    FC experiment of HeLa using 12250-1-AP (same clone as 12250-1-PBS)

    1X10^6 HeLa cells were intracellularly stained with 0.2 ug Anti-Human Calpastatin (12250-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.2 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer. This data was developed using the same antibody clone with 12250-1-PBS in a different storage buffer formulation.

Calpastatin Polyclonal antibody, PBS Only

Calpastatin Polyclonal Antibody for WB, IHC, IF/ICC, FC (Intra), IP, Indirect ELISA
Cat No. 12250-1-PBS

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IHC, IF/ICC, FC (Intra), IP, Indirect ELISA

别名

CAST, Calpain inhibitor, Sperm BS-17 component

缓冲液配方: 
PBS, Azide, Glycerol
偶联物:  Unconjugated
规格价格库存

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产品信息

12250-1-PBS targets Calpastatin in WB, IHC, IF/ICC, FC (Intra), IP, Indirect ELISA applications and shows reactivity with human samples.

经测试应用 WB, IHC, IF/ICC, FC (Intra), IP, Indirect ELISA Application Description
经测试反应性 human
免疫原

CatNo: Ag2897

Product name: Recombinant human CAST protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-350 aa of BC013579

Sequence: MNPTETKAVKTEPEKKSQSTKPKSLPKQASDTGSNDAHNKKAVSRSAEQQPSEKSTEPKTKPQDMISAGGESVAGITAISGKPGDKKKEKKSLTPAVPVESKPDKPSGKSGMDAALDDLIDTLGGPEETEEENTTYTGPEVSDPMSSTYIEELGKREVTIPPKYRELLAKKEGITGPPADSSKPIGPDDAIDALSSDFTCGSPTAAGKKTEKEESTEVLKAQSAGTVRSAAPPQEKKRKVEKDTMSDQALEALSASLGTRQAEPELDLRSIKEVDEAKAKEEKLEKCGEDDETIPSEYRLKPATDKDGKPLLPEPEEKPKPRSESELIDELSEDFDRSECKEKPSKPTEK

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 calpastatin
别名 CAST, Calpain inhibitor, Sperm BS-17 component
计算分子量 667 aa, 72 kDa
观测分子量 110 kDa
GenBank蛋白编号BC013579
基因名称 Calpastatin
Gene ID (NCBI) 831
RRIDAB_2071702
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDP20810
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Calpastatin (CAST) is an endogenous protein that specifically inhibits the activity of calpains, a family of calcium-dependent proteases involved in numerous cellular processes. The CAST gene encodes calpastatin, which contains multiple calpain-inhibiting domains that bind to the active site of calpain, preventing it from performing its functions. Calpastatin is found in the cytosol and plays a key role in regulating intracellular proteolysis, with malfunctions in the calpain-calpastatin system linked to various diseases, including autoimmune disorders and neurodegenerative conditions.

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