验证数据展示
经过测试的应用
| Positive WB detected in | Jurkat cells, MCF-7 cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
33924-1-AP targets Calpain 15/SOLH in WB, ELISA applications and shows reactivity with human samples.
| 经测试应用 | WB, ELISA Application Description |
| 经测试反应性 | human |
| 免疫原 |
CatNo: Ag40689 Product name: Recombinant human SOLH protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-150 aa of BC021854 Sequence: MATVGEWSCVRCTFLNPAGQRQCSICEAPRHKPDLNHILRLSVEEQKWPCARCTFRNFLGKEACEVCGFTPEPAPGAAFLPVLNGVLPKPPAILGEPKGSCQEEAGPVRTAGLVATEPARGQCEDKDEEEKEEQEEEEGAAEPRGGWACP 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | small optic lobes homolog (Drosophila) |
| 别名 | Calpain 15, Calpain15, Calpain-15, CAPN15, EC:3.4.22.- |
| 计算分子量 | 117 kDa |
| 观测分子量 | 117 kDa |
| GenBank蛋白编号 | BC021854 |
| 基因名称 | SOLH |
| Gene ID (NCBI) | 6650 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity Purification |
| UNIPROT ID | O75808 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Calpain 15 (also known as CAPN15 and SOLH) is an intracellular cysteine protease belonging to the non-classical small optic lobe (SOL) family of calpains, which plays an important role in developmental processes (PMID: 40238785). Capn15 is expressed at much higher levels during early neuronal development than in the adult (PMID: 32885237). And loss of Capn15 in forebrain excitatory neurons leads to some behavioral deficits including decreased fear generalization (PMID: 37598906).
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for Calpain 15/SOLH antibody 33924-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
