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验证数据展示

  • WB analysis using 28270-1-AP (same clone as 28270-1-PBS)

    WB analysis using 28270-1-AP (same clone as 28270-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 28270-1-AP (Calmodulin 1/2/3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 28270-1-PBS in a different storage buffer formulation.

  • IHC staining of rat brain using 28270-1-AP (same clone as 28270-1-PBS)

    IHC staining of rat brain using 28270-1-AP (same clone as 28270-1-PBS)

    Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 28270-1-AP (Calmodulin 1/2/3 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 28270-1-PBS in a different storage buffer formulation.

  • IHC staining of rat brain using 28270-1-AP (same clone as 28270-1-PBS)

    IHC staining of rat brain using 28270-1-AP (same clone as 28270-1-PBS)

    Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 28270-1-AP (Calmodulin 1/2/3 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 28270-1-PBS in a different storage buffer formulation.

  • IHC staining of mouse brain using 28270-1-AP (same clone as 28270-1-PBS)

    IHC staining of mouse brain using 28270-1-AP (same clone as 28270-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 28270-1-AP (Calmodulin 1/2/3 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 28270-1-PBS in a different storage buffer formulation.

  • IF Staining of HeLa using 28270-1-AP (same clone as 28270-1-PBS)

    IF Staining of HeLa using 28270-1-AP (same clone as 28270-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using Calmodulin 1/2/3 antibody (28270-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 28270-1-PBS in a different storage buffer formulation.

Calmodulin 1/2/3 Polyclonal antibody, PBS Only

Calmodulin 1/2/3 Polyclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA
Cat No. 28270-1-PBS

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, IF/ICC, Indirect ELISA

别名

CALM2, CALM, CALM1, CALM3, CALML2

缓冲液配方:  PBS Only
PBS, Azide, Glycerol
偶联物:  Unconjugated
规格价格库存

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产品信息

28270-1-PBS targets Calmodulin 1/2/3 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IF/ICC, Indirect ELISA Application Description
经测试反应性 human, mouse, rat
免疫原

CatNo: Ag28000

Product name: Recombinant human CALM2 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 72-149 aa of BC006464

Sequence: MMARKMKDTDSEEEIREAFRVFDKDGNGYISAAELRHVMTNLGEKLTDEEVDEMIREADIDGDGQVNYEEFVQMMTAK

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 calmodulin 2 (phosphorylase kinase, delta)
别名 CALM2, CALM, CALM1, CALM3, CALML2
计算分子量 17 kDa
观测分子量 17-22 kDa
GenBank蛋白编号BC006464
基因名称 Calmodulin 1
Gene ID (NCBI) 805
RRIDAB_3669650
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDP62158
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Calmodulin is the most widely distributed and most versatile member of a family of calcium-binding proteins which probably serve as receptors for the Ca2+ signal. Through the regulation of a wide variety of intracellular enzymes, calmodulin plays a key role in many physiological processes (PMID: 6313166).

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