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验证数据展示

  • WB analysis using 28270-1-AP

    WB analysis using 28270-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 28270-1-AP (Calmodulin 1/2/3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IHC staining of rat brain using 28270-1-AP

    IHC staining of rat brain using 28270-1-AP

    Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 28270-1-AP (Calmodulin 1/2/3 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of rat brain using 28270-1-AP

    IHC staining of rat brain using 28270-1-AP

    Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 28270-1-AP (Calmodulin 1/2/3 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of mouse brain using 28270-1-AP

    IHC staining of mouse brain using 28270-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 28270-1-AP (Calmodulin 1/2/3 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of HeLa using 28270-1-AP

    IF Staining of HeLa using 28270-1-AP

    Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using Calmodulin 1/2/3 antibody (28270-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

Calmodulin 1/2/3 Polyclonal antibody

Calmodulin 1/2/3 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 28270-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, IF/ICC, ELISA

别名

CALM2, CALM, CALM1, CALM3, CALML2

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
偶联物:  Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inmouse skeletal muscle tissue, rat skeletal muscle tissue
Positive IHC detected inrat brain tissue, mouse brain tissue, mouse skeletal muscle tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHeLa cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

28270-1-AP targets Calmodulin 1/2/3 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IF/ICC, ELISA Application Description
经测试反应性 human, mouse, rat
免疫原

CatNo: Ag28000

Product name: Recombinant human CALM2 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 72-149 aa of BC006464

Sequence: MMARKMKDTDSEEEIREAFRVFDKDGNGYISAAELRHVMTNLGEKLTDEEVDEMIREADIDGDGQVNYEEFVQMMTAK

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 calmodulin 2 (phosphorylase kinase, delta)
别名 CALM2, CALM, CALM1, CALM3, CALML2
计算分子量 17 kDa
观测分子量 17-22 kDa
GenBank蛋白编号BC006464
基因名称 Calmodulin 1
Gene ID (NCBI) 805
RRIDAB_3669650
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDP62158
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Calmodulin is the most widely distributed and most versatile member of a family of calcium-binding proteins which probably serve as receptors for the Ca2+ signal. Through the regulation of a wide variety of intracellular enzymes, calmodulin plays a key role in many physiological processes (PMID: 6313166).

实验方案

Product Specific Protocols
IF protocol for Calmodulin 1/2/3 antibody 28270-1-APDownload protocol
IHC protocol for Calmodulin 1/2/3 antibody 28270-1-APDownload protocol
WB protocol for Calmodulin 1/2/3 antibody 28270-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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