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验证数据展示

  • FC experiment of SH-SY5Y using CL488-13730

    FC experiment of SH-SY5Y using CL488-13730

    1x10^6 SH-SY5Y cells were intracellularly stained with 0.8 ug CoraLite® Plus 488 Anti-Human CaMKII alpha (CL488-13730) (red), or 0.8 ug CoraLite® Plus 488-conjugated Rabbit IgG control Rabbit PolyAb (CL488-30000, Clone: ) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer.

  • IF Staining of SH-SY5Y using CL488-13730

    IF Staining of SH-SY5Y using CL488-13730

    Immunofluorescent analysis of (-20°C Ethanol) fixed SH-SY5Y cells using CoraLite® Plus 488 CaMKII alpha antibody (CL488-13730) at dilution of 1:200.

CoraLite® Plus 488-conjugated CaMKII alpha Polyclonal antibody

CaMKII alpha Polyclonal Antibody for IF/ICC, FC (Intra)
Cat No. CL488-13730

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

IF/ICC, FC (Intra)

别名

CAMK2A, Calcium/calmodulin-dependent protein kinase type II subunit alpha, CAMK2, CAMKA, CaMK-II subunit alpha

缓冲液配方:  PBS, Proclin 300, BSA, Glycerol
PBS, Proclin 300, BSA, Glycerol
偶联物:  CoraLite® Plus 488
规格价格库存

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经过测试的应用

Positive IF/ICC detected inSH-SY5Y cells
Positive FC (Intra) detected inSH-SY5Y cells
Planning an IHC experiment? We recommend our IHCeasy® CAMK2A Ready-To-Use IHC Kit. CAMK2A primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 13730-1-AP

推荐稀释比

应用推荐稀释比
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.80 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-13730 targets CaMKII alpha in IF/ICC, FC (Intra) applications and shows reactivity with human, mouse, rat samples.

经测试应用 IF/ICC, FC (Intra) Application Description
经测试反应性 human, mouse, rat
免疫原

CatNo: Ag4745

Product name: Recombinant human CAMK2A protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-271 aa of BC040457

Sequence: MATITCTRFTEEYQLFEELGKGAFSVVRRCVKVLAGQEYAAKIINTKKLSARDHQKLEREARICRLLKHPNIVRLHDSISEEGHHYLIFDLVTGGELFEDIVAREYYSEADASHCIQQILEAVLHCHQMGVVHRDLKPENLLLASKLKGAAVKLADFGLAIEVEGEQQAWFGFAGTPGYLSPEVLRKDPYGKPVDLWACGVILYILLVGYPPFWDEDQHRLYKQIKAGAYDFPSPEWDTVTPEAKDLINKMLTINPSKRITAAEALKHPWI

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 calcium/calmodulin-dependent protein kinase II alpha
别名 CAMK2A, Calcium/calmodulin-dependent protein kinase type II subunit alpha, CAMK2, CAMKA, CaMK-II subunit alpha
计算分子量 478 aa, 54 kDa
观测分子量 45-60 kDa
GenBank蛋白编号BC040457
基因名称 CaMKII alpha
Gene ID (NCBI) 815
RRIDAB_3672572
偶联类型 CoraLite® Plus 488 Fluorescent Dye
最大激发/发射波长493 nm / 522 nm
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ9UQM7
储存缓冲液 PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
储存条件Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

CaM-kinase II (CAMK2) is a prominent kinase in the central nervous system that may function in long-term potentiation and neurotransmitter release. It is a member of the NMDAR signaling complex in excitatory synapses it may regulate NMDAR-dependent potentiation of the AMPAR and synaptic plasticity. This antibody can recognize all the members of CAMK2 family.

实验方案

Product Specific Protocols
FC protocol for CL Plus 488 CaMKII alpha antibody CL488-13730Download protocol
IF protocol for CL Plus 488 CaMKII alpha antibody CL488-13730Download protocol
Standard Protocols
Click here to view our Standard Protocols
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