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验证数据展示

  • WB analysis using 13243-1-AP

    WB analysis using 13243-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 13243-1-AP (CYP4F12 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

  • IHC staining of human stomach cancer using 13243-1-AP

    IHC staining of human stomach cancer using 13243-1-AP

    Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 13243-1-AP (CYP4F12 antibody) at dilution of 1:800 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human small intestine using 13243-1-AP

    IHC staining of human small intestine using 13243-1-AP

    Immunohistochemical analysis of paraffin-embedded human small intestine tissue slide using 13243-1-AP (CYP4F12 antibody) at dilution of 1:500 (under 40x lens).

CYP4F12 Polyclonal antibody

CYP4F12 Polyclonal Antibody for WB, IHC, ELISA
Cat No. 13243-1-AP

产品说明书

引用文献(1)

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, ELISA

别名

CYPIVF12, Cytochrome P450 4F12, EC:1.14.14.1, UNQ568/PRO1129

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inmouse liver tissue, human placenta tissue, rat liver tissue
Positive IHC detected inhuman stomach cancer tissue, human small intestine tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:1000-1:8000
Immunohistochemistry (IHC)IHC : 1:400-1:1600
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 1 publications below

产品信息

13243-1-AP targets CYP4F12 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, ELISA Application Description
文献引用应用WB
经测试反应性 human, mouse, rat
文献引用反应性human
免疫原 CYP4F12 fusion protein Ag3957 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 cytochrome P450, family 4, subfamily F, polypeptide 12
别名 CYPIVF12, Cytochrome P450 4F12, EC:1.14.14.1, UNQ568/PRO1129
计算分子量 524 aa, 60 kDa
观测分子量 50-60 kDa
GenBank蛋白编号BC035350
基因名称 CYP4F12
Gene ID (NCBI) 66002
RRIDAB_2088412
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ9HCS2
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

CYP4F12(Cytochrome P450 4F12) Catalyzes leukotriene B4 omega-hydroxylation and arachidonic acid omega-hydroxylation but with an activity much lower than that of CYP4F2. It expresses a band of 60KDa in adult and fetal liver, kidney, placenta at term, seminal vesicles, and the prostate gland(PMID:15078342). And it can be phosphorylated in vivo(PMID:18828626).

实验方案

Product Specific Protocols
WB protocol for CYP4F12 antibody 13243-1-APDownload protocol
IHC protocol for CYP4F12 antibody 13243-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

Sci Rep

CYP4F12 is a potential biomarker and inhibits cell migration of head and neck squamous cell carcinoma via EMT pathway

Authors - Wenming Jia
View Article