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CYP4B1 Polyclonal antibody

CYP4B1 Polyclonal Antibody for IHC, IP, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

IHC, IP, WB,ELISA

Conjugate

Unconjugated

Cat No : 11771-1-AP

Print datasheet

Synonyms

CYP4B1, CYPIVB1, Cytochrome P450 4B1, Cytochrome P450 HP, P 450HP

验证数据展示

  • WB analysis using 11771-1-AP

    WB analysis using 11771-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 11771-1-AP (CYP4B1 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

  • IP experiment of mouse lung using 11771-1-AP

    IP experiment of mouse lung using 11771-1-AP

    IP result of anti-CYP4B1 (IP:11771-1-AP, 4ug; Detection:11771-1-AP 1:500) with mouse lung tissue lysate 4000ug.

  • IHC staining of human lung cancer using 11771-1-AP

    IHC staining of human lung cancer using 11771-1-AP

    Immunohistochemical analysis of paraffin-embedded human lung cancer using 11771-1-AP (CYP4B1 antibody) at dilution of 1:50 (under 10x lens).

经过测试的应用

Positive WB detected inmouse lung tissue, A549 cells, rat kidney tissue
Positive IP detected inmouse lung tissue
Positive IHC detected inhuman lung cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1500
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

IHCSee 1 publications below

产品信息

11771-1-AP targets CYP4B1 in WB, IP, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications IHC, IP, WB,ELISA
Cited ApplicationsIHC
Tested Reactivity human, mouse, rat
Cited Reactivityhuman
Immunogen CYP4B1 fusion protein Ag2416 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name cytochrome P450, family 4, subfamily B, polypeptide 1
Synonyms CYP4B1, CYPIVB1, Cytochrome P450 4B1, Cytochrome P450 HP, P 450HP
Calculated Molecular Weight 512 aa, 59 kDa
Observed Molecular Weight 55-59 kDa
GenBank Accession NumberBC017758
Gene Symbol CYP4B1
Gene ID (NCBI) 1580
RRIDAB_11182944
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP13584
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for CYP4B1 antibody 11771-1-APDownload protocol
IHC protocol for CYP4B1 antibody 11771-1-APDownload protocol
IP protocol for CYP4B1 antibody 11771-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanIHC

Hum Reprod

Single-cell profiling reveals mechanisms of uncontrolled inflammation and glycolysis in decidual stromal cell subtypes in recurrent miscarriage

Authors - Shihua Bao
View Article