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验证数据展示

  • WB analysis using 11771-1-AP

    WB analysis using 11771-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 11771-1-AP (CYP4B1 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

  • IP experiment of mouse lung using 11771-1-AP

    IP experiment of mouse lung using 11771-1-AP

    IP result of anti-CYP4B1 (IP:11771-1-AP, 4ug; Detection:11771-1-AP 1:500) with mouse lung tissue lysate 4000ug.

  • IHC staining of human lung cancer using 11771-1-AP

    IHC staining of human lung cancer using 11771-1-AP

    Immunohistochemical analysis of paraffin-embedded human lung cancer using 11771-1-AP (CYP4B1 antibody) at dilution of 1:50 (under 10x lens).

CYP4B1 Polyclonal antibody

CYP4B1 Polyclonal Antibody for WB, IP, IHC, ELISA
Cat No. 11771-1-AP

产品说明书

引用文献(1)

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IP, IHC, ELISA

别名

CYP4B1, CYPIVB1, Cytochrome P450 4B1, Cytochrome P450 HP, P 450HP

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inmouse lung tissue, A549 cells, rat kidney tissue
Positive IP detected inmouse lung tissue
Positive IHC detected inhuman lung cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:1500
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

IHCSee 1 publications below

产品信息

11771-1-AP targets CYP4B1 in WB, IP, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IP, IHC, ELISA Application Description
文献引用应用IHC
经测试反应性 human, mouse, rat
文献引用反应性human
免疫原 CYP4B1 fusion protein Ag2416 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 cytochrome P450, family 4, subfamily B, polypeptide 1
别名 CYP4B1, CYPIVB1, Cytochrome P450 4B1, Cytochrome P450 HP, P 450HP
计算分子量 512 aa, 59 kDa
观测分子量 55-59 kDa
GenBank蛋白编号BC017758
基因名称 CYP4B1
Gene ID (NCBI) 1580
RRIDAB_11182944
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDP13584
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for CYP4B1 antibody 11771-1-APDownload protocol
IHC protocol for CYP4B1 antibody 11771-1-APDownload protocol
IP protocol for CYP4B1 antibody 11771-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanIHC

Hum Reprod

Single-cell profiling reveals mechanisms of uncontrolled inflammation and glycolysis in decidual stromal cell subtypes in recurrent miscarriage

Authors - Shihua Bao
View Article