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验证数据展示

  • WB analysis using 86957-1-RR (same clone as 86957-1-PBS)

    WB analysis using 86957-1-RR (same clone as 86957-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 86957-1-RR (CYP46A1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 86957-1-PBS in a different storage buffer formulation.

  • IHC staining of mouse brain using 86957-1-RR (same clone as 86957-1-PBS)

    IHC staining of mouse brain using 86957-1-RR (same clone as 86957-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 86957-1-RR (CYP46A1 antibody) at dilution of 1:3000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 86957-1-PBS in a different storage buffer formulation.

  • IHC staining of mouse brain using 86957-1-RR (same clone as 86957-1-PBS)

    IHC staining of mouse brain using 86957-1-RR (same clone as 86957-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 86957-1-RR (CYP46A1 antibody) at dilution of 1:3000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 86957-1-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 86957-1-RR

    Affinity and Kinetic Characterization of 86957-1-RR

    Biolayer interferometry (BLl) kinetic assays of 86957-1-RR against Human CYP46A1 were performed. The affinity constant is 6.95 nM.

CYP46A1 Recombinant monoclonal antibody, PBS Only

CYP46A1 Uni-rAb® Recombinant Antibody for WB, IHC, Indirect ELISA
Cat No. 86957-1-PBS

产品说明书

CloneNo. 250155G8

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, Indirect ELISA

别名

CH24H, Cholesterol 24 hydroxylase, Cholesterol 24-hydroxylase, Cholesterol 24-monooxygenase, Cholesterol 24S-hydroxylase

缓冲液配方:  PBS Only
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
规格价格库存

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产品信息

86957-1-PBS targets CYP46A1 in WB, IHC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, Indirect ELISA Application Description
经测试反应性 human, mouse, rat
免疫原

CatNo: Ag16786

Product name: Recombinant human CYP46A1 protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 201-500 aa of BC022539

Sequence: TSMLLGAQKPLSQAVKLMLEGITASRNTLAKFLPGKRKQLREVRESIRFLRQVGRDWVQRRREALKRGEEVPADILTQILKAEEGAQDDEGLLDNFVTFFIAGHETSANHLAFTVMELSRQPEIVARLQAEVDEVIGSKRYLDFEDLGRLQYLSQVLKESLRLYPPAWGTFRLLEEETLIDGVRVPGNTPLLFSTYVMGRMDTYFEDPLTFNPDRFGPGAPKPRFTYFPFSLGHRSCIGQQFAQMEVKVVMAKLLQRLEFRLVPGQRFGLQEQATLKPLDPVLCTLRPRGWQPAPPPPPC

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 cytochrome P450, family 46, subfamily A, polypeptide 1
别名 CH24H, Cholesterol 24 hydroxylase, Cholesterol 24-hydroxylase, Cholesterol 24-monooxygenase, Cholesterol 24S-hydroxylase
计算分子量 500 aa, 57 kDa
观测分子量 57 kDa
GenBank蛋白编号BC022539
基因名称 CYP46A1
Gene ID (NCBI) 10858
RRIDAB_3745233
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purification
UNIPROT IDQ9Y6A2
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Cytochrome P450 46A1 (CYP46A1), also named as CYP46, P450 46A1, CH24H and Cholesterol 24-hydroxylase, belongs to the cytochrome P450 family. CYP46A1 is a central nervous system-specific enzyme, and can freely cross the blood-brain barrier and be degraded in the liver. It is involved in the turnover of cholesterol. It converts cholesterol into 24S-hydroxycholesterol and, to a lesser extent, 25-hydroxycholesterol. CYP46A1 is significantly upregulated in hepatic lymphomyeloid cells compared with ESCs.(PMID:20039312)

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