验证数据展示
经过测试的应用
| Positive WB detected in | mouse liver tissue, HepG2 cells, mouse lung tissue, rat liver tissue |
| Positive IHC detected in | human liver tissue, human liver cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:2000-1:10000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| WB | See 5 publications below |
| IHC | See 1 publications below |
产品信息
21721-1-AP targets CYP2A6 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB, IHC, ELISA Application Description |
| 文献引用应用 | WB, IHC |
| 经测试反应性 | human, mouse, rat |
| 文献引用反应性 | human, mouse |
| 免疫原 |
CatNo: Ag16377 Product name: Recombinant human CYP2A6 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 198-494 aa of BC096253 Sequence: FLSLLRMMLGIFQFTSTSTGQLYEMFSSVMKHLPGPQQQAFQLLQGLEDFIAKKVEHNQRTLDPNSPRDFIDSFLIRMQEEEKNPNTEFYLKNLVMTTLNLFIGGTETVSTTLRYGFLLLMKHPEVEAKVHEEIDRVIGKNRQPKFEDRAKMPYMEAVIHEIQRFGDVIPMSLARRVKKDTKFRDFFLPKGTEVYPMLGSVLRDPSFFSNPQDFNPQHFLNEKGQFKKSDAFVPFSIGKRNCFGEGLARMELFLFFTTVMQNFRLKSSQSPKDIDVSPKHVGFATIPRNYTMSFLPR 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | cytochrome P450, family 2, subfamily A, polypeptide 6 |
| 别名 | Coumarin 7 hydroxylase, CYP2A, CYP2A3, CYP2A6, CYPIIA6, Cytochrome P450 2A6, Cytochrome P450 IIA3, Cytochrome P450(I), P450C2A, P450PB |
| 计算分子量 | 494 aa, 57 kDa |
| 观测分子量 | 50 kDa |
| GenBank蛋白编号 | BC096253 |
| 基因名称 | CYP2A6 |
| Gene ID (NCBI) | 1548 |
| RRID | AB_10791886 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | P11509 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
实验方案
| Product Specific Protocols | |
|---|---|
| IHC protocol for CYP2A6 antibody 21721-1-AP | Download protocol |
| WB protocol for CYP2A6 antibody 21721-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Biochem Pharmacol Drug resistance induces the upregulation of H2S-producing enzymes in HCT116 colon cancer cells. | ||
Xenobiotica Comparison of mouse and human cytochrome P450 mediated-drug metabolising activities in hepatic and extrahepatic microsomes. | ||
Sci Rep Extracellular vesicles released from macrophages modulates interleukin-1β in astrocytic and neuronal cells | ||
Front Toxicol E-cigarette exposure causes early pro-atherogenic changes in an inducible murine model of atherosclerosis | ||
Biochem Biophys Res Commun Circadian rhythms in CYP2A5 expression underlie the time-dependent effect of tegafur on breast cancer | ||
Biochem Biophys Res Commun Circadian rhythms in CYP2A5 expression underlie the time-dependent effect of tegafur on breast cancer |
