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验证数据展示

  • WB analysis of Caco-2 using 67045-1-Ig (same clone as 67045-1-PBS)

    WB analysis of Caco-2 using 67045-1-Ig (same clone as 67045-1-PBS)

    Caco-2 cells were subjected to SDS PAGE followed by western blot with 67045-1-Ig (CYP27A1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67045-1-PBS in a different storage buffer formulation.

  • WB analysis of HepG2 using 67045-1-Ig (same clone as 67045-1-PBS)

    WB analysis of HepG2 using 67045-1-Ig (same clone as 67045-1-PBS)

    WB result of CYP27A1 antibody (67045-1-Ig; 1:10000; incubated at room temperature for 1.5 hours) with sh-Control and sh-CYP27A1 transfected HepG2 cells. This data was developed using the same antibody clone with 67045-1-PBS in a different storage buffer formulation.

  • IHC staining of human liver cancer using 67045-1-Ig (same clone as 67045-1-PBS)

    IHC staining of human liver cancer using 67045-1-Ig (same clone as 67045-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 67045-1-Ig (CYP27A1 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67045-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with CYP27A1 antibody included
  • IHC staining of human liver cancer using 67045-1-Ig (same clone as 67045-1-PBS)

    IHC staining of human liver cancer using 67045-1-Ig (same clone as 67045-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 67045-1-Ig (CYP27A1 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67045-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with CYP27A1 antibody included
  • IF Staining of human liver cancer using 67045-1-Ig (same clone as 67045-1-PBS)

    IF Staining of human liver cancer using 67045-1-Ig (same clone as 67045-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed human liver cancer tissue using CYP27A1 antibody (67045-1-Ig, Clone: 3G3F7 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67045-1-PBS in a different storage buffer formulation.

  • IF Staining of human liver cancer using 67045-1-Ig (same clone as 67045-1-PBS)

    IF Staining of human liver cancer using 67045-1-Ig (same clone as 67045-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed human liver cancer tissue using CYP27A1 antibody (67045-1-Ig, Clone: 3G3F7 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67045-1-PBS in a different storage buffer formulation.

  • Featured Product
  • KD/KO验证

CYP27A1 Monoclonal antibody, PBS Only

CYP27A1 Monoclonal Antibody for WB, IHC, IF-P, ELISA
Cat No. 67045-1-PBS

产品说明书

CloneNo. 3G3F7

宿主/亚型

Mouse / IgG1

种属反应性

human, mouse, rat

应用

WB, IHC, IF-P, ELISA

别名

3G3F7, 5-beta-cholestane-3-alpha,7-alpha,12-alpha-triol 26-hydroxylase, CP27, CTX, CYP27

缓冲液配方:  PBS Only
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
规格价格库存

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Planning an IHC experiment? We recommend our IHCeasy CYP27A1 Ready-To-Use IHC Kit. CYP27A1 primary antibody included.

产品信息

67045-1-PBS targets CYP27A1 in WB, IHC, IF-P, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IF-P, ELISA Application Description
经测试反应性 human, mouse, rat
免疫原

CatNo: Ag7746

Product name: Recombinant human CYP27A1 protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 183-531 aa of BC051851

Sequence: DDFMTRLDQLRAESASGNQVSDMAQLFYYFALEAICYILFEKRIGCLQRSIPEDTVTFVRSIGLMFQNSLYATFLPKWTRPVLPFWKRYLDGWNAIFSFGKKLIDEKLEDMEAQLQAAGPDGIQVSGYLHFLLASGQLSPREAMGSLPELLMAGVDTTSNTLTWALYHLSKDPEIQEALHEEVVGVVPAGQVPQHKDFAHMPLLKAVLKETLRLYPVVPTNSRIIEKEIEVDGFLFPKNTQFVFCHYVVSRDPTAFSEPESFQPHRWLRNSQPATPRIQHPFGSVPFGYGVRACLGRRIAELEMQLLLARLIQKYKVVLAPETGELKSVARIVLVPNKKVGLQFLQRQC

种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 cytochrome P450, family 27, subfamily A, polypeptide 1
别名 3G3F7, 5-beta-cholestane-3-alpha,7-alpha,12-alpha-triol 26-hydroxylase, CP27, CTX, CYP27
计算分子量 60 kDa
观测分子量 54-60 kDa
GenBank蛋白编号BC051851
基因名称 CYP27A1
Gene ID (NCBI) 1593
RRIDAB_2882359
偶联类型 Unconjugated
形式Liquid
纯化方式Protein G purification
UNIPROT IDQ02318
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C
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