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CYP27A1 Monoclonal antibody

CYP27A1 Monoclonal Antibody for IF-P, IHC, WB, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, Mouse, Rat and More (2)

Applications

IF-P, IHC, WB, ELISA

Conjugate

Unconjugated

CloneNo.

3G3F7

Cat No : 67045-1-Ig

Print datasheet

Synonyms

CP27, CTX, CYP27, CYP27A1, Cytochrome P 450C27/25, Cytochrome P450 27, Sterol 27 hydroxylase, Vitamin D(3) 25 hydroxylase

验证数据展示

  • WB analysis of Caco-2 using 67045-1-Ig

    WB analysis of Caco-2 using 67045-1-Ig

    Caco-2 cells were subjected to SDS PAGE followed by western blot with 67045-1-Ig (CYP27A1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • WB analysis of HepG2 using 67045-1-Ig

    WB analysis of HepG2 using 67045-1-Ig

    WB result of CYP27A1 antibody (67045-1-Ig; 1:10000; incubated at room temperature for 1.5 hours) with sh-Control and sh-CYP27A1 transfected HepG2 cells.

  • IHC staining of human liver cancer using 67045-1-Ig

    IHC staining of human liver cancer using 67045-1-Ig

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 67045-1-Ig (CYP27A1 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with CYP27A1 antibody included
  • IHC staining of human liver cancer using 67045-1-Ig

    IHC staining of human liver cancer using 67045-1-Ig

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 67045-1-Ig (CYP27A1 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with CYP27A1 antibody included
  • IF Staining of human liver cancer using 67045-1-Ig

    IF Staining of human liver cancer using 67045-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed human liver cancer tissue using CYP27A1 antibody (67045-1-Ig, Clone: 3G3F7 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

  • IF Staining of human liver cancer using 67045-1-Ig

    IF Staining of human liver cancer using 67045-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed human liver cancer tissue using CYP27A1 antibody (67045-1-Ig, Clone: 3G3F7 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

经过测试的应用

Positive WB detected inCaco-2 cells, HepG2 cells, HT-29 cells, rat liver tissue
Positive IHC detected inhuman liver cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inhuman liver cancer tissue
Planning an IHC experiment? We recommend our IHCeasy CYP27A1 Ready-To-Use IHC Kit. CYP27A1 primary antibody included.
Planning an IF experiment? We recommend our CoraLite®594 conjugated versions of this antibody.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
Immunohistochemistry (IHC)IHC : 1:250-1:1000
Immunofluorescence (IF)IF : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 3 publications below

产品信息

67045-1-Ig targets CYP27A1 in WB, IF, IHC, ELISA applications and shows reactivity with Human, Mouse, Rat samples.

Tested Applications IF-P, IHC, WB, ELISA
Cited ApplicationsWB
Tested Reactivity Human, Mouse, Rat
Cited Reactivityhuman, mouse
Immunogen CYP27A1 fusion protein Ag7746 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name cytochrome P450, family 27, subfamily A, polypeptide 1
Synonyms CP27, CTX, CYP27, CYP27A1, Cytochrome P 450C27/25, Cytochrome P450 27, Sterol 27 hydroxylase, Vitamin D(3) 25 hydroxylase
Calculated Molecular Weight 60 kDa
Observed Molecular Weight 54-60 kDa
GenBank Accession NumberBC051851
Gene Symbol CYP27A1
Gene ID (NCBI) 1593
RRIDAB_2882359
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
UNIPROT IDQ02318
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for CYP27A1 antibody 67045-1-IgDownload protocol
IHC protocol for CYP27A1 antibody 67045-1-IgDownload protocol
IF protocol for CYP27A1 antibody 67045-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
mouseWB

J Ethnopharmacol

Penthorum chinense Pursh. extract attenuates non-alcholic fatty liver disease by regulating gut microbiota and bile acid metabolism in mice.

Authors - Xiaoxi Li
View Article
mouseWB

Nat Microbiol

Akkermansia muciniphila protects mice against an emerging tick-borne viral pathogen

Authors - Jinyan Xie
View Article
humanWB

Front Immunol

Construction of a lipid metabolism-related risk model for hepatocellular carcinoma by single cell and machine learning analysis

Authors - Lisha Mou
View Article