验证数据展示
经过测试的应用
| Positive WB detected in | Caco-2 cells, HepG2 cells, HT-29 cells, rat liver tissue |
| Positive IHC detected in | human liver cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF-P detected in | human liver cancer tissue |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:2000-1:10000 |
| Immunohistochemistry (IHC) | IHC : 1:250-1:1000 |
| Immunofluorescence (IF)-P | IF-P : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
67045-1-Ig targets CYP27A1 in WB, IHC, IF-P, ELISA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB, IHC, IF-P, ELISA Application Description |
| 文献引用应用 | WB, IHC, IF |
| 经测试反应性 | human, mouse, rat |
| 文献引用反应性 | human, mouse, rat |
| 免疫原 |
CatNo: Ag7746 Product name: Recombinant human CYP27A1 protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 183-531 aa of BC051851 Sequence: DDFMTRLDQLRAESASGNQVSDMAQLFYYFALEAICYILFEKRIGCLQRSIPEDTVTFVRSIGLMFQNSLYATFLPKWTRPVLPFWKRYLDGWNAIFSFGKKLIDEKLEDMEAQLQAAGPDGIQVSGYLHFLLASGQLSPREAMGSLPELLMAGVDTTSNTLTWALYHLSKDPEIQEALHEEVVGVVPAGQVPQHKDFAHMPLLKAVLKETLRLYPVVPTNSRIIEKEIEVDGFLFPKNTQFVFCHYVVSRDPTAFSEPESFQPHRWLRNSQPATPRIQHPFGSVPFGYGVRACLGRRIAELEMQLLLARLIQKYKVVLAPETGELKSVARIVLVPNKKVGLQFLQRQC 种属同源性预测 |
| 宿主/亚型 | Mouse / IgG1 |
| 抗体类别 | Monoclonal |
| 产品类型 | Antibody |
| 全称 | cytochrome P450, family 27, subfamily A, polypeptide 1 |
| 别名 | 3G3F7, 5-beta-cholestane-3-alpha,7-alpha,12-alpha-triol 26-hydroxylase, CP27, CTX, CYP27 |
| 计算分子量 | 60 kDa |
| 观测分子量 | 54-60 kDa |
| GenBank蛋白编号 | BC051851 |
| 基因名称 | CYP27A1 |
| Gene ID (NCBI) | 1593 |
| RRID | AB_2882359 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Protein G purification |
| UNIPROT ID | Q02318 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
实验方案
| Product Specific Protocols | |
|---|---|
| IF protocol for CYP27A1 antibody 67045-1-Ig | Download protocol |
| IHC protocol for CYP27A1 antibody 67045-1-Ig | Download protocol |
| WB protocol for CYP27A1 antibody 67045-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
J Bone Miner Res CYP4A22 Loss-of-Function Causes A New Type of Vitamin D-dependent Rickets (VDDR1C) | ||
J Ethnopharmacol Penthorum chinense Pursh. extract attenuates non-alcholic fatty liver disease by regulating gut microbiota and bile acid metabolism in mice. | ||
Nat Microbiol Akkermansia muciniphila protects mice against an emerging tick-borne viral pathogen | ||
Front Immunol Construction of a lipid metabolism-related risk model for hepatocellular carcinoma by single cell and machine learning analysis | ||
Adv Sci (Weinh) Geniposidic Acid Targeting FXR "S332 and H447" Mediated Conformational Change to Upregulate CYPs and miR-19a-3p to Ameliorate Drug-Induced Liver Injury | ||
J Ethnopharmacol Integration of network pharmacology, UHPLC-Q exactive orbitrap HRMS technique and metabolomics to elucidate the active ingredients and mechanisms of compound danshen pills in treating hypercholesterolemic rats |
