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  • Featured Product
  • KD/KO Validated

CoraLite®594-conjugated CYB5R3 Polyclonal antibody

CYB5R3 Polyclonal Antibody for FC (Intra), IF/ICC

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, pig, rat

Applications

FC (Intra), IF/ICC

Conjugate

CoraLite®594 Fluorescent Dye

Cat No : CL594-10894

Print datasheet

Synonyms

B5R, CYB5R3, Cytochrome b5 reductase, cytochrome b5 reductase 3, DIA1, Diaphorase 1, NADH cytochrome b5 reductase 3

验证数据展示

  • IF Staining of HeLa using CL594-10894

    IF Staining of HeLa using CL594-10894

    Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using CoraLite®594 CYB5R3 antibody (CL594-10894) at dilution of 1:200.

  • FC experiment of HeLa using CL594-10894

    FC experiment of HeLa using CL594-10894

    1X10^6 HeLa cells were intracellularly stained with 0.4 ug CoraLite®594 Anti-Human CYB5R3 (CL594-10894) (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

经过测试的应用

Positive IF detected inHeLa cells
Positive FC detected inHeLa cells
Planning an IHC experiment? We recommend our IHCeasy CYB5R3 Ready-To-Use IHC Kit. CYB5R3 primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 10894-1-AP

推荐稀释比

ApplicationDilution
Immunofluorescence (IF)IF : 1:50-1:500
Flow Cytometry (FC)FC : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL594-10894 targets CYB5R3 in IF, FC (Intra) applications and shows reactivity with human, mouse, pig, rat samples.

Tested Applications FC (Intra), IF/ICC
Tested Reactivity human, mouse, pig, rat
Immunogen CYB5R3 fusion protein Ag1339 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name cytochrome b5 reductase 3
Synonyms B5R, CYB5R3, Cytochrome b5 reductase, cytochrome b5 reductase 3, DIA1, Diaphorase 1, NADH cytochrome b5 reductase 3
Calculated Molecular Weight 33 kDa
Observed Molecular Weight 30-35 kDa
GenBank Accession NumberBC004821
Gene Symbol CYB5R3
Gene ID (NCBI) 1727
RRIDAB_2919751
Conjugate CoraLite®594 Fluorescent Dye
Excitation/Emission Maxima Wavelengths588 nm / 604 nm
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP00387
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

CYB5R3, also named as DIA1 and B5R, belongs to the flavoprotein pyridine nucleotide cytochrome reductase family. It plays an essential role in the mitochondrial metabolism by its contribution to cellular redox homeostasis. Overstimulation of CYB5R3 associated with lipid rafts can account for the overshot of plasma membrane-focalized superoxide anion production that triggers the entry of CGN in the irreversible phase of apoptosis.

实验方案

Product Specific Protocols
IF protocol for CL594 CYB5R3 antibody CL594-10894Download protocol
FC protocol for CL594 CYB5R3 antibody CL594-10894Download protocol
Standard Protocols
Click here to view our Standard Protocols