验证数据展示
经过测试的应用
| Positive WB detected in | HeLa cells, HEK-293 cells, mouse kidney tissue |
| Positive IP detected in | mouse kidney tissue |
| Positive IF/ICC detected in | HeLa cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:20-1:200 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| WB | See 4 publications below |
| IP | See 1 publications below |
产品信息
24021-1-AP targets CXorf56 in WB, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse samples.
| 经测试应用 | WB, IF/ICC, IP, ELISA Application Description |
| 文献引用应用 | WB, IP |
| 经测试反应性 | human, mouse |
| 文献引用反应性 | human, monkey |
| 免疫原 |
CatNo: Ag19856 Product name: Recombinant human CXorf56 protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 1-222 aa of BC023506 Sequence: MPKVVSRSVVCSDTRDREEYDDGEKPLHVYYCLCGQMVLVLDCQLEKLPMRPRDRSRVIDAAKHAHKFCNTEDEETMYLRRPEGIERQYRKKCAKCGLPLFYQSQPKNAPVTFIVDGAVVKFGQGFGKTNIYTQKQEPPKKVMMTKRTKDMGKFSSVTVSTIDEEEEEIEAREVADSYAQNAKVIEKQLERKGMSKRRLQELAELEAKKAKMKGTLIDNQFK 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | chromosome X open reading frame 56 |
| 别名 | CXorf56, STEEP, STING ER exit protein, UPF0428 protein CXorf56 |
| 计算分子量 | 222 aa, 26 kDa |
| 观测分子量 | 26-30 kDa |
| GenBank蛋白编号 | BC023506 |
| 基因名称 | CXorf56 |
| Gene ID (NCBI) | 63932 |
| RRID | AB_2879404 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen Affinity purified |
| UNIPROT ID | Q9H5V9 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
CxORF56 (also known as STEEP) is an evolutionarily highly conserved protein whose function is unknown. Recently CxORF56 has been identified as a protein that interacts with STING, and it is essential for STING ER exit. CxORF56-dependent STING ER exit is essential for STING signaling in antiviral defense and in inflammatory diseases.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for CXorf56 antibody 24021-1-AP | Download protocol |
| IF protocol for CXorf56 antibody 24021-1-AP | Download protocol |
| IP protocol for CXorf56 antibody 24021-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
EBioMedicine A STING antagonist modulating the interaction with STIM1 blocks ER-to-Golgi trafficking and inhibits lupus pathology. | ||
J Virol The Papain-Like Protease of Porcine Reproductive and Respiratory Syndrome Virus Impedes STING Translocation from the Endoplasmic Reticulum to the Golgi Apparatus by Deubiquitinating STIM1 | ||
NPJ Breast Cancer Inhibition of CXorf56 promotes PARP inhibitor-induced cytotoxicity in triple-negative breast cancer | ||
Nat Commun Cholesterol-binding motifs in STING that control endoplasmic reticulum retention mediate anti-tumoral activity of cholesterol-lowering compounds |
