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验证数据展示

  • WB analysis of mouse brain using 16513-1-AP (same clone as 16513-1-PBS)

    WB analysis of mouse brain using 16513-1-AP (same clone as 16513-1-PBS)

    mouse brain tissue were subjected to SDS PAGE followed by western blot with 16513-1-AP (CXXC5 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 16513-1-PBS in a different storage buffer formulation.

  • WB analysis using 16513-1-AP (same clone as 16513-1-PBS)

    WB analysis using 16513-1-AP (same clone as 16513-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 16513-1-AP (CXXC5 antibody) at dilution of 1:30000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 16513-1-PBS in a different storage buffer formulation.

  • IP experiment of SH-SY5Y using 16513-1-AP (same clone as 16513-1-PBS)

    IP experiment of SH-SY5Y using 16513-1-AP (same clone as 16513-1-PBS)

    IP result of anti-CXXC5 (IP:16513-1-AP, 4ug; Detection:16513-1-AP 1:6000) with SH-SY5Y cells lysate 1280 ug. This data was developed using the same antibody clone with 16513-1-PBS in a different storage buffer formulation.

  • IP experiment of SH-SY5Y using 16513-1-AP (same clone as 16513-1-PBS)

    IP experiment of SH-SY5Y using 16513-1-AP (same clone as 16513-1-PBS)

    IP result of anti-CXXC5 (IP:16513-1-AP, 4ug; Detection:16513-1-AP 1:300) with SH-SY5Y cells lysate 3400 ug. This data was developed using the same antibody clone with 16513-1-PBS in a different storage buffer formulation.

  • IHC staining of human prostate cancer using 16513-1-AP (same clone as 16513-1-PBS)

    IHC staining of human prostate cancer using 16513-1-AP (same clone as 16513-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 16513-1-AP (CXXC5 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 16513-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with CXXC5 antibody included
  • IP experiment of mouse brain using 16513-1-AP (same clone as 16513-1-PBS)

    IP experiment of mouse brain using 16513-1-AP (same clone as 16513-1-PBS)

    IP result of anti-CXXC5 (IP:16513-1-AP, 4ug; Detection:16513-1-AP 1:6000) with mouse brain tissue lysate 2400 ug. This data was developed using the same antibody clone with 16513-1-PBS in a different storage buffer formulation.

  • IHC staining of mouse brain using 16513-1-AP (same clone as 16513-1-PBS)

    IHC staining of mouse brain using 16513-1-AP (same clone as 16513-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 16513-1-AP (CXXC5 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 16513-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with CXXC5 antibody included
  • IHC staining of mouse brain using 16513-1-AP (same clone as 16513-1-PBS)

    IHC staining of mouse brain using 16513-1-AP (same clone as 16513-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 16513-1-AP (CXXC5 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 16513-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with CXXC5 antibody included
  • IHC staining of rat brain using 16513-1-AP (same clone as 16513-1-PBS)

    IHC staining of rat brain using 16513-1-AP (same clone as 16513-1-PBS)

    Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 16513-1-AP (CXXC5 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 16513-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with CXXC5 antibody included
  • IF Staining of MCF-7 using 16513-1-AP (same clone as 16513-1-PBS)

    IF Staining of MCF-7 using 16513-1-AP (same clone as 16513-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed MCF-7 cells using CXXC5 antibody (16513-1-AP) at dilution of 1:200 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002), CL594-phalloidin (red). This data was developed using the same antibody clone with 16513-1-PBS in a different storage buffer formulation.

  • WB analysis of MCF-7 using 16513-1-AP (same clone as 16513-1-PBS)

    WB analysis of MCF-7 using 16513-1-AP (same clone as 16513-1-PBS)

    WB result of CXXC5 antibody (16513-1-AP; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-CXXC5 transfected MCF-7 cells. This data was developed using the same antibody clone with 16513-1-PBS in a different storage buffer formulation.

  • Featured Product
  • KD/KO Validated

CXXC5 Polyclonal antibody, PBS Only

CXXC5 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA
Cat No. 16513-1-PBS

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, IF/ICC, IP, ELISA

别名

CF5, CXXC finger 5, CXXC-type zinc finger protein 5, HSPC195, Putative MAPK-activating protein PM08

缓冲液配方: 
PBS, Azide, Glycerol
偶联物:  Unconjugated
规格价格库存

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Planning an IHC experiment? We recommend our IHCeasy CXXC5 Ready-To-Use IHC Kit. CXXC5 primary antibody included.

产品信息

16513-1-PBS targets CXXC5 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IF/ICC, IP, ELISA Application Description
经测试反应性 human, mouse, rat
免疫原

CatNo: Ag9733

Product name: Recombinant human CXXC5 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-227 aa of BC017439

Sequence: MMGGESADKATAAAAAASLLANGHDLAAAMAVDKSNPTSKHKSGAVASLLSKAERATELAAEGQLTLQQFAQSTEMLKRVVQEHLPLMSEAGAGLPDMEAVAGAEALNGQSDFPYLGAFPINPGLFIMTPAGVFLAESALHMAGLAEYPMQGELASAISSGKKKRKRCGMCAPCRRRINCEQCSSCRNRKTGHQICKFRKCEELKKKPSAALEKVMLPTGAAFRWFQ

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 CXXC finger 5
别名 CF5, CXXC finger 5, CXXC-type zinc finger protein 5, HSPC195, Putative MAPK-activating protein PM08
计算分子量322aa,33 kDa; 227aa,24 kDa
观测分子量 35-40 kDa
GenBank蛋白编号BC017439
基因名称 CXXC5
Gene ID (NCBI) 51523
RRIDAB_2878269
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ7LFL8
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

CXXC5 (CXXC-type zinc finger protein 5) is also named as HSPC195, RINF and TCCCIA00297.CXXC5 is a member of the CXXC-type zinc-finger protein family. CXXC5 is a short protein (322 amino acids in length) that does not have any catalytic domain, but is able to bind to DNA and act as a transcription factor and epigenetic factor through protein-protein interactions. In addition, CXXC5 is capable of regulating various signal transduction processes, including the TGF-β, Wnt and ATM-p53 pathways, thereby acting as a novel and crucial signaling coordinator. CXXC5 plays an important role in embryonic development and adult tissue homeostasis by regulating cell proliferation, differentiation and apoptosis (PMID: 30479059). CXXC5 has two isoforms of 33 kDa and 24 kDa (PMID: 25805812). CXXC5 is modified by phosphorylation after translation.

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