验证数据展示

WB analysis of mouse brain using 16513-1-AP
mouse brain tissue were subjected to SDS PAGE followed by western blot with 16513-1-AP (CXXC5 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.
WB analysis using 16513-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 16513-1-AP (CXXC5 antibody) at dilution of 1:30000 incubated at room temperature for 1.5 hours.
IP experiment of SH-SY5Y using 16513-1-AP
IP result of anti-CXXC5 (IP:16513-1-AP, 4ug; Detection:16513-1-AP 1:6000) with SH-SY5Y cells lysate 1280 ug.
IP experiment of SH-SY5Y using 16513-1-AP
IP result of anti-CXXC5 (IP:16513-1-AP, 4ug; Detection:16513-1-AP 1:300) with SH-SY5Y cells lysate 3400 ug.
IHC staining of human prostate cancer using 16513-1-AP
Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 16513-1-AP (CXXC5 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
Ready-to-use IHC kits available with CXXC5 antibody included
IP experiment of mouse brain using 16513-1-AP
IP result of anti-CXXC5 (IP:16513-1-AP, 4ug; Detection:16513-1-AP 1:6000) with mouse brain tissue lysate 2400 ug.
IHC staining of mouse brain using 16513-1-AP
Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 16513-1-AP (CXXC5 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
Ready-to-use IHC kits available with CXXC5 antibody included
IHC staining of mouse brain using 16513-1-AP
Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 16513-1-AP (CXXC5 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
Ready-to-use IHC kits available with CXXC5 antibody included
IHC staining of rat brain using 16513-1-AP
Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 16513-1-AP (CXXC5 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
Ready-to-use IHC kits available with CXXC5 antibody included
IF Staining of MCF-7 using 16513-1-AP
Immunofluorescent analysis of (4% PFA) fixed MCF-7 cells using CXXC5 antibody (16513-1-AP) at dilution of 1:200 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002), CL594-phalloidin (red).

Featured Product
KD/KO验证

CXXC5 Polyclonal antibody

CXXC5 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Cat No. 16513-1-AP

产品说明书

引用文献(11)

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat and More (1)

应用

WB, IHC, IF/ICC, IP, ELISA and More (1)

别名

CF5, CXXC finger 5, CXXC-type zinc finger protein 5, HSPC195, Putative MAPK-activating protein PM08

缓冲液配方: PBS, Azide, Glycerol

PBS, Azide, Glycerol

偶联物: Unconjugated

Unconjugated

规格	价格	库存

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概述

经过测试的应用
产品信息
发表文章 (11)
实验方案

产品说明书

MSDS

经过测试的应用

Positive WB detected in	MCF-7 cells, mouse brain tissue, SH-SY5Y cells
Positive IP detected in	SH-SY5Y cells, mouse brain tissue
Positive IHC detected in	mouse brain tissue, human prostate cancer tissue, rat brain tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***
Positive IF/ICC detected in	MCF-7 cells

Planning an IHC experiment? We recommend our IHCeasy CXXC5 Ready-To-Use IHC Kit. CXXC5 primary antibody included.

应用	推荐稀释比
Western Blot (WB)	WB : 1:5000-1:50000
Immunoprecipitation (IP)	IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)	IHC : 1:200-1:800
Immunofluorescence (IF)/ICC	IF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

KD/KO	See 3 publications below
WB	See 10 publications below
IHC	See 1 publications below
ChIP	See 4 publications below

产品信息

16513-1-AP targets CXXC5 in WB, IHC, IF/ICC, IP, ChIP, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用	WB, IHC, IF/ICC, IP, ELISA Application Description
文献引用应用	WB, IHC, ChIP
经测试反应性	human, mouse, rat
文献引用反应性	human, mouse, zebrafish
免疫原	CatNo: Ag9733 Product name: Recombinant human CXXC5 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-227 aa of BC017439 Sequence: MMGGESADKATAAAAAASLLANGHDLAAAMAVDKSNPTSKHKSGAVASLLSKAERATELAAEGQLTLQQFAQSTEMLKRVVQEHLPLMSEAGAGLPDMEAVAGAEALNGQSDFPYLGAFPINPGLFIMTPAGVFLAESALHMAGLAEYPMQGELASAISSGKKKRKRCGMCAPCRRRINCEQCSSCRNRKTGHQICKFRKCEELKKKPSAALEKVMLPTGAAFRWFQ 种属同源性预测
宿主/亚型	Rabbit / IgG
抗体类别	Polyclonal
产品类型	Antibody
全称	CXXC finger 5
别名	CF5, CXXC finger 5, CXXC-type zinc finger protein 5, HSPC195, Putative MAPK-activating protein PM08
计算分子量	322aa,33 kDa; 227aa,24 kDa
观测分子量	35-40 kDa
GenBank蛋白编号	BC017439
基因名称	CXXC5
Gene ID (NCBI)	51523
RRID	AB_2878269
偶联类型	Unconjugated
形式	Liquid
纯化方式	Antigen affinity purification
UNIPROT ID	Q7LFL8
储存缓冲液	PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

背景介绍

CXXC5 (CXXC-type zinc finger protein 5) is also named as HSPC195, RINF and TCCCIA00297.CXXC5 is a member of the CXXC-type zinc-finger protein family. CXXC5 is a short protein (322 amino acids in length) that does not have any catalytic domain, but is able to bind to DNA and act as a transcription factor and epigenetic factor through protein-protein interactions. In addition, CXXC5 is capable of regulating various signal transduction processes, including the TGF-β, Wnt and ATM-p53 pathways, thereby acting as a novel and crucial signaling coordinator. CXXC5 plays an important role in embryonic development and adult tissue homeostasis by regulating cell proliferation, differentiation and apoptosis (PMID: 30479059). CXXC5 has two isoforms of 33 kDa and 24 kDa (PMID: 25805812). CXXC5 is modified by phosphorylation after translation.

实验方案

Product Specific Protocols
IF protocol for CXXC5 antibody 16513-1-AP	Download protocol
IHC protocol for CXXC5 antibody 16513-1-AP	Download protocol
IP protocol for CXXC5 antibody 16513-1-AP	Download protocol
WB protocol for CXXC5 antibody 16513-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

发表文章

Species	Application	Title
human	WB,IHC,ChIP	Nat Commun A noncanonical AR addiction drives enzalutamide resistance in prostate cancer. Authors - Yundong He KD Validated View Article
mouse	WB	J Exp Med Epigenetic regulator CXXC5 recruits DNA demethylase Tet2 to regulate TLR7/9-elicited IFN response in pDCs. Authors - Shixin Ma KO Validated View Article
human	WB	Oncogene NUDT21 negatively regulates PSMB2 and CXXC5 by alternative polyadenylation and contributes to hepatocellular carcinoma suppression. Authors - Sheng Tan KD Validated View Article
human	WB,ChIP	Haematologica The epigenetic regulator RINF (CXXC5) maintains SMAD7 expression in human immature erythroid cells and sustains red blood cells expansion. Authors - Audrey Astori View Article
human	WB	Sci Rep KANK1 inhibits cell growth by inducing apoptosis though regulating CXXC5 in human malignant peripheral nerve sheath tumors. Authors - Zhibin Cui View Article
mouse,human	WB,ChIP	Front Cell Dev Biol Down-Regulation of CXXC5 De-Represses MYCL1 to Promote Hepatic Stellate Cell Activation. Authors - Xiaoyan Wu View Article

验证数据展示

WB analysis of mouse brain using 16513-1-AP

WB analysis using 16513-1-AP

IP experiment of SH-SY5Y using 16513-1-AP

IP experiment of SH-SY5Y using 16513-1-AP

IHC staining of human prostate cancer using 16513-1-AP

IP experiment of mouse brain using 16513-1-AP

IHC staining of mouse brain using 16513-1-AP

IHC staining of mouse brain using 16513-1-AP

IHC staining of rat brain using 16513-1-AP

IF Staining of MCF-7 using 16513-1-AP

CXXC5 Polyclonal antibody

CXXC5 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

经过测试的应用

推荐稀释比

发表文章中的应用

产品信息

背景介绍

实验方案

发表文章

A noncanonical AR addiction drives enzalutamide resistance in prostate cancer.

Epigenetic regulator CXXC5 recruits DNA demethylase Tet2 to regulate TLR7/9-elicited IFN response in pDCs.

NUDT21 negatively regulates PSMB2 and CXXC5 by alternative polyadenylation and contributes to hepatocellular carcinoma suppression.

The epigenetic regulator RINF (CXXC5) maintains SMAD7 expression in human immature erythroid cells and sustains red blood cells expansion.

KANK1 inhibits cell growth by inducing apoptosis though regulating CXXC5 in human malignant peripheral nerve sheath tumors.

Down-Regulation of CXXC5 De-Represses MYCL1 to Promote Hepatic Stellate Cell Activation.